Inosine Fluorometric Assay Kit (BN00932)

(No reviews yet) Write a Review
SKU:
BN00932
€613

Description

ELISA Kit Technical ManualMSDS

Inosine Fluorometric Assay Kit

Inosine is commonly found in tRNAs and is essential for proper translation of the genetic code in wobble base pairs. Knowledge of inosine metabolism has led to advances in immunotherapy in recent decades. Inosine and hypoxanthine are also potential markers of ATP catabolic-by-products from oxidative stress which follows acute cardiac ischemia. In Assay Genie's Inosine Assay Kit, inosine is converted to hypoxanthine, which will react with the substrate mix and to generate fluorescence (Ex/Em = 535/587 nm) in presence of converter and developer enzymes. This assay has a detection limit of of approximately 100 pmol inosine/well.

Figure: Fluorometric Standard Curve obtained using the kit protocol

Key Information Description

Product SKU

BN00932

Size

100 assays

Detection Method

Fluorescence (Ex/Em = 535/587 nm)

Species Reactivity

Mammalian

Applications

This assay has a detection limit of of approximately 100 pmol inosine/well.

Features and Benefits

  • Simple procedure; takes ~ less than 40 minutes
  • Fast and convenient

Kit Components

  • Inosine Assay Buffer
  • Converter Enzyme
  • Developer Enzyme
  • Inosine Substrate Mix
  • Inosine Standard (10 mM)

Storage Conditions

-20°C

Shipping Conditions

Gel Pack

USAGE

For Research Use Only! Not For Use in Humans.

View AllClose