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Lactate Colorimetric Assay Kit II (BN00851)

SKU:
BN00851
Product type:
Cellular Assay
  • Cell Biology Assays Lactate Colorimetric Assay Kit II BN00851
  • Cell Biology Assays Lactate Colorimetric Assay Kit II BN00851
€732

Description

ELISA Kit Technical ManualMSDS

Lactate Colorimetric Assay Kit II

Lactate (CHH(OH)COO-) plays important roles in many biological processes. Abnormally high concentrations of lactate have been related to disease states such as diabetes and lactic acidosis, etc. L(+)-Lactate is the major lactate stereoisomer formed in human intermediary metabolism and is present in blood. D(-)-Lactate is also present but only at about 1-5% of the concentration of L(+)-Lactate. In the Lactate Assay Kit, lactate is oxidized by lactate dehydrogenase to generate a product which interacts with a probe to produce a color (ax = 450 nm). The kit detect L(+)-Lactate in biological samples such as in serum or plasma, cells, culture and fermentation media. There is no need for pretreatment or purification of samples. It detects 0.02 mM - 10 mM lactate in various samples.

Figure:  (a)Lactate Standard Curve (b) Lactate estimation in pooled human Serum and yogurt. Undiluted serum was used in the assay as described in the kit protocol. Deproteinizing Sample Preparation Kit (Cat. #BN01024) was used to remove protein from the yogurt and the supernatant was diluted 1:200 - 1:500. Assay was performed following the kit protocol.

Key Information Description

Product SKU

BN00851

Size

100 assays

Detection Method

Absorbance (450 nm)

Species Reactivity

Mammalian

Applications

Kit detects 0.02 mM - 10 mM lactate in various samples.

Features and Benefits

  • Simple procedure; takes ~40 minutes
  • Fast and convenient
  • Kit contains the necessary reagents for accurate measurement of Lactate

Kit Components

  • Lactate Assay Buffer
  • Lactate Enzyme Mix
  • L(+)-Lactate Standard (100 mM)

Storage Conditions

-20°C

Shipping Conditions

Gel Pack

USAGE

For Research Use Only! Not For Use in Humans.