Lactate Fluorometric Assay Kit (BN00862)

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SKU:
BN00862
  • Lactate Fluorometric Assay Kit (BN00862)
  • Lactate Fluorometric Assay Kit (BN00862)
€708

Description

ELISA Kit Technical ManualMSDS

Lactate Fluorometric Assay Kit

Lactate (CH3CH(OH)COO-) plays an important role in many biological processes. Abnormally high concentrations of lactate have been related to diseases such as diabetes, lactic acidosis, etc. L(+)-Lactate is present in blood and is the major lactate stereoisomer formed in human intermediary metabolism. D-Lactate is also present but only at about 1-5% of L(+)-Lactate concentration. Lactate is a significant energy source for living organisms and can be used to generate cellular ATP. Assay Genie's Lactate Assay kit is suitable for measuring very low levels of L(+)-lactate in a variety of samples. In this assay, L(+)-lactate is specifically oxidized to form an intermediate that reacts with a colorless probe to generate fluorescence (Ex/Em = 535/587 nm), which is directly proportional to the amount of lactate. This simple, rapid & high-throughput suitable assay kit is the most sensitive Lactate Assay kit on the market. It can detect L(+)-lactate less than 0.2 µM in a variety of biological samples.

Figure: (a) Lactate Standard Curve (b) Measurement of Lactate levels in rat liver (1.2 µg), kidney (0.7 µg) ; muscle (0.45 µg) in human serum (0.5 µl from 1:10 diluted serum) . Assays were performed following kit protocol.

Key Information Description

Product SKU

BN00862

Size

100 assays

Detection Method

Fluorescence (Ex/Em= 535/587 nm)

Applications

  • The assay can detect L(+)-lactate less than 0.2 µM in a variety of biological samples.
  • Analysis of metabolism and cell signaling.
  • Mechanistic study for diabetes.

Features and Benefits

  • Simple, rapid, and high-throughput adaptable.
  • Kit is the most sensitive Lactate Assay kit on the market.

Kit Components

  • Lactate Assay Buffer (in DMSO)
  • Lactate Enzyme Mix (lyophilized)
  • Lactate Substrate Mix (lyophilized)
  • L(+)-Lactate Standard (100 mM)

Storage Conditions

-20°C

Shipping Conditions

Gel Pack

USAGE

For Research Use Only! Not For Use in Humans.

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