Lipase Activity Fluorometric Assay Kit III (BN00943)

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BN00943
  • Lipase Activity Fluorometric Assay Kit III (BN00943)
  • Lipase Activity Fluorometric Assay Kit III (BN00943)
€613

Description

ELISA Kit Technical ManualMSDS

Lipase Activity Fluorometric Assay Kit III

Lipase is a subclass of the esterases that catalyze the hydrolysis of ester bonds of lipid substrates. Lipases perform essential roles in the digestion, transport and processing of dietary lipids (e.g. triglycerides, fats, oils) in most, if not all, living organisms. In humans, pancreatic lipase is the key enzyme responsible for breaking down fats in the digestive system by converting triglycerides to monoglycerides and free fatty acids. During the damage of the pancreas, lipase levels can rise 5 to 10-fold within 24 to 48 hours. In Assay Genie's Lipase Assay Kit III, Lipase hydrolyzes a specific substrate to generate the methylresorufin, which can be detected fluorometrically at Em/Ex=529/600 nm. The kit provides a rapid, simple, more sensitive, and reliable test suitable for high throughput assay of Lipase activity. This kit can be used to detect Lipase as low as 0.1 microU/well.

Figure: Standard Curve and Positive Control and background graph

Key Information Description

Product SKU

BN00943

Size

100 assays

Detection Method

Fluorescence (Ex/Em 529/600 nm)

Species Reactivity

Mammalian

Applications

A sensitive and high speed homogeneous assay for detection of Lipase enzyme activity in a high-throughput screening (HTS) format.

Features and Benefits

  • Simple procedure; takes ~ 60 minutes
  • Fast and convenient
  • Sensitive assays for measuring Lipase activity as low as 0.1 mU/well.

Kit Components

  • Lipase Assay Buffer
  • Lipase Substrate
  • Methylresorufin Standard (0.1 mM)
  • Lipase Positive Control

Storage Conditions

-20°C

Shipping Conditions

Gel Pack

USAGE

For Research Use Only! Not For Use in Humans.

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