Malate Dehydrogenase Activity Colorimetric Assay Kit (BN00878)

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SKU:
BN00878
  • Malate Dehydrogenase Activity Colorimetric Assay Kit (BN00878)
  • Malate Dehydrogenase Activity Colorimetric Assay Kit (BN00878)
  • Malate Dehydrogenase Activity Colorimetric Assay Kit (BN00878)
€655

Description

ELISA Kit Technical ManualMSDS

Malate Dehydrogenase Activity Colorimetric Assay Kit

Malate Dehydrogenase (MDH) (EC 1.1.1.37) is an important enzyme which reversibly converts L-malate into oxaloacetate in the presence of NAD. In eukaryotic cells, malate dehydrogenase has 2 isoforms: MDH1 and MDH2. MDH1 is cytosolic & participates in the malate-aspartate shuttle, which transports malate into mitochondria for utilization in ATP generation whereas MDH2 is a mitochondrial enzyme and part of the citric acid cycle. MDH activity is increased in some neurodegenerative diseases such as Alzheimer's disease, and abnormal MDH activity in serum can serve as a diagnostic tool for severe liver damage (e.g. Hepatocellular carcinoma). In Assay Genie's Malate Dehydrogenase Activity Assay kit, MDH reacts with malate to form an intermediate. The generated intermediate reacts with MDH Developer to form a colored product with strong absorbance at 450 nm. The assay is simple, sensitive and can detect less than 0.5 mU of MDH activity in various sample types.

Figure: (a) NADH Standard Curve (b) Malate Dehydrogenase activity in rat muscle extract (0.8 µg), Jurkat cell lysate (0.6 µg), yeast mitochondria (1.2 μg) and MDH Positive Control (c) Referenced MDH Activity in Jurkat cell lysate, rat muscle extract and yeast mitochondrial lysate Assays were performed following the kit protocol.

Key Information Description

Product SKU

BN00878

Size

100 assays

Detection Methods

Absorbance (450 nm)

Applications

  • Measurement of malate dehydrogenase activity in various tissues/cells
  • Analysis of citric acid cycle and malate-asparate shuttle

Features and Benefits

  • Simple, rapid & convenient
  • The assay can detect Malate Dehydrogenase (MDH) Activity less than 0.5 mU in various sample types.

Kit Components

  • MDH Assay Buffer
  • MDH Substrate (Lyophilized)
  • MDH Enzyme Mix (Lyophilized)
  • MDH Developer (Lyophilized)
  • NADH Standard (Lyophilized)
  • MDH Positive Control (Lyophilized)

Storage Conditions

-20°C

Shipping Conditions

Gel Pack

USAGE

For Research Use Only! Not For Use in Humans.

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