Mitochondrial Permeability Transition Pore Assay Kit (BN00507)

SKU:
BN00507
€625

Description

ELISA Kit Technical ManualMSDS

Mitochondrial Permeability Transition Pore Assay Kit

Mitochondria are the power centrals of the cell and play an essential role in energy production. Damage to mitochondria activates signaling pathways that induce apoptosis. The mitochondrial permeability transition pore (MPT pore or MPTP) is a nonspecific channel formed by components of the inner and outer mitochondrial membranes, and appears to be involved in the release of mitochondrial components during cell death. In healthy cells, MPTP's flicker between open and closed states but during cell death MPTP's dramatically alter the permeability of the mitochondria. Cytochrome c release and loss of mitochondrial membrane potential are subsequent to continuous pore activation. Assay Genie's Mitochondrial Permeability Transition Pore Assay Kit provides a direct method of measuring cell death by measuring MPTP opening rather than relying on mitochondrial membrane potential alone.

Figure 1: Jurkat cells were incubated with the reagents of the Mitochondrial Permeability Transition Pore Assay Kit and analyzed by flow cytometer. Tube 1: sample without treatment - used for instrument setup; Tube 2: sample stained with MPTP Staining Dye showing cumulative fluorescence signal from both cytoplasm and mitochondria; Tube 3: sample stained with MPTP Staining Dye and treated with CoCl 2 showing mitochondrial fluorescence only; Tube 4: samples with all reagents showing the lowest fluorescence. The difference in fluorescence between tubes 3 and 4 indicates the degree of MPTP activation and subsequent depolarization of the mitochondrial membrane.

Key Information Description

Product SKU

BN00507

Size

100 assays

Detection Method

Flow cytometry (Ex 488 nm)

Species Reactivity

Mammalian

Applications

  • Measure mitochondrial permeability transition pore activity in cells
  • Assessment of physiological mediators and antibodies that affect cell viability/cytotoxicity

Features and Benefits

  • Simple procedure
  • Fast, convenient and sensitive
  • The assay provides a direct method of measuring mitochondrial permeability transition pore activation as an early marker of apoptosis

Kit Components

  • MPTP Staining Dye
  • CoCl2
  • Ionomycin
  • MPTP Wash Buffer

Storage Conditions

-20°C

Shipping Conditions

Gel Pack

USAGE

For Research Use Only! Not For Use in Humans.

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Additional Information

Product type:
Cellular Assay
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