NADH Fluorometric Assay Kit (BN00604)

SKU:
BN00604
  • Cell Biology Assays NADH Fluorometric Assay Kit BN00604
  • Cell Biology Assays NADH Fluorometric Assay Kit BN00604
  • Cell Biology Assays NADH Fluorometric Assay Kit BN00604
€661

Description

ELISA Kit Technical ManualMSDS

NADH Fluorometric Assay Kit

NAD and NADH exist in all living organisms and are important for energy transformation and maintenance of the redox state of cells or tissues. The reduced form, NADH is a driving force for cells and plays a critical role in cell regulation and repair processes. It is of increasing interest to be able to measure low level of NADH in samples or in enzymatic reactions. Assay Genie's NADH Assay kit provides a highly sensitive method to detect low level of NADH (NAD is not detected in the reaction). There is no requirement to purify NADH from samples. The NADH Recycling Enzyme Mix specifically recognizes NADH (not NADPH) in the enzyme recycling reaction. The assay is rapid, simple and can measure less than 8 nM NADH in a variety of samples.

Figure: (a) NADH Standard Curve (b) Decomposition of NAD, but not NADH (c) Measurememts of NADH in Rat Kidney (1.74µg) muscle (2.51µg), heart (1.59µg) and liver lysates (29.5µg)

Key Information Description

Product SKU

BN00604

Size

100 assays

Detection Methods

Fluorescence (Ex/Em=535/587 nm)

Applications

  • Measurement of NADH in various tissues/cells.
  • Measurement of low levels of NADH in enzymatic reactions.
  • Analysis of metabolism and cell signaling in various cells

Features and Benefits

  • Highly sensitive method to detect low levels of NADH.
  • Simple, fast and convenient.
  • This assay can measure less than 8 nM NADH in a variety of samples.

Kit Compositions

  • NADH Extraction Buffer
  • NADH Cycling Buffer (DMSO)
  • NAD Cycling Enzyme Mix (Lyophilized)
  • NADH Standard (Lyophilized)

Storage Conditions

-20°C

Shipping Conditions

Gel Pack

USAGE

For Research Use Only! Not For Use in Humans

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Additional Information

Product type:
Cellular Assay
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