The NSMCE2 Polyclonal Antibody (PACO38498) is a crucial tool for researchers studying NSMCE2, a protein involved in DNA repair and maintenance. This antibody, generated in rabbits, exhibits high specificity and sensitivity towards NSMCE2 in human samples, making it ideal for a variety of applications, including Western blotting.NSMCE2, also known as Structural Maintenance of Chromosomes 4 (SMC4) E3, plays a vital role in maintaining genomic stability by ensuring proper DNA replication and repair processes. Dysregulation of NSMCE2 has been linked to various diseases, including cancer and genetic disorders.
By studying the function and expression of NSMCE2 using this antibody, researchers can gain valuable insights into the molecular mechanisms underlying these conditions.With its reliability and versatility, the NSMCE2 Polyclonal Antibody (PACO38498) is a valuable tool for researchers conducting studies in genetics, cancer biology, and DNA repair pathways. By facilitating the detection and analysis of NSMCE2, this antibody contributes to the advancement of scientific knowledge and the development of potential therapeutic interventions targeting NSMCE2-related diseases.
Antibody Name:
NSMCE2 Antibody (PACO38498)
Antibody SKU:
PACO38498
Size:
50ug
Host Species:
Rabbit
Tested Applications:
ELISA, IHC
Recommended Dilutions:
ELISA:1:2000-1:10000, IHC:1:500-1:1000
Species Reactivity:
Human
Immunogen:
Recombinant Human E3 SUMO-protein ligase NSE2 protein (1-247AA)
IHC image of PACO38498 diluted at 1:600 and staining in paraffin-embedded human melanoma performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Background:
E3 SUMO-protein ligase component of the SMC5-SMC6 complex, a complex involved in DNA double-strand break repair by homologous recombination. Is not be required for the stability of the complex. The complex may promote sister chromatid homologous recombination by recruiting the SMC1-SMC3 cohesin complex to double-strand breaks. The complex is required for telomere maintenance via recombination in ALT (alternative lengthening of telomeres) cell lines and mediates sumoylation of shelterin complex (telosome) components which is proposed to lead to shelterin complex disassembly in ALT-associated PML bodies (APBs). Acts as a E3 ligase mediating SUMO attachment to various proteins such as SMC6L1 and TRAX, the shelterin complex subunits TERF1, TERF2, TINF2 and TERF2IP, and maybe the cohesin components RAD21 and STAG2. Required for recruitment of telomeres to PML nuclear bodies. SUMO protein-ligase activity is required for the prevention of DNA damage-induced apoptosis by facilitating DNA repair, and for formation of APBs in ALT cell lines. Required for sister chromatid cohesion during prometaphase and mitotic progression.
Synonyms:
E3 SUMO-protein ligase NSE2 (EC 2.3.2.-) (E3 SUMO-protein transferase NSE2) (MMS21 homolog) (hMMS21) (Non-structural maintenance of chromosomes element 2 homolog) (Non-SMC element 2 homolog), NSMCE2, C8orf36 MMS21
UniProt Protein Function:
NSMCE2: E3 SUMO-protein ligase component of the SMC5-SMC6 complex, a complex involved in DNA double-strand break repair by homologous recombination. Is not be required for the stability of the complex. The complex may promote sister chromatid homologous recombination by recruiting the SMC1-SMC3 cohesin complex to double-strand breaks. The complex is required for telomere maintenance via recombination in ALT (alternative lengthening of telomeres) cell lines and mediates sumoylation of shelterin complex (telosome) components which is proposed to lead to shelterin complex disassembly in ALT-associated PML bodies (APBs). Acts as a E3 ligase mediating SUMO attachment to various proteins such as SMC6L1 and TRAX, the shelterin complex subunits TERF1, TERF2, TINF2 and TERF2IP, and maybe the cohesin components RAD21 and STAG2. Required for recruitment of telomeres to PML nuclear bodies. SUMO protein-ligase activity is required for the prevention of DNA damage-induced apoptosis by facilitating DNA repair, and for formation of APBs in ALT cell lines. Required for sister chromatid cohesion during prometaphase and mitotic progression. Belongs to the NSE2 family.Protein type: Ubiquitin ligase; SUMO conjugating system; EC 6.-.-.-; EC 6.3.2.-; LigaseChromosomal Location of Human Ortholog: 8q24.13Cellular Component: PML body; chromosome, telomeric region; nucleusMolecular Function: protein binding; zinc ion binding; SUMO ligase activity; ligase activityBiological Process: mitosis; positive regulation of maintenance of mitotic sister chromatid cohesion; protein sumoylation; cell division; telomere maintenance via recombination; positive regulation of mitotic metaphase/anaphase transition; double-strand break repair via homologous recombination; double-strand break repair via nonhomologous end joining
UniProt Protein Details:
NCBI Summary:
This gene encodes a member of a family of E3 small ubiquitin-related modifier (SUMO) ligases that mediates the attachment of a SUMO protein to proteins involved in nuclear transport, transcription, chromosome segregation and DNA repair. The encoded protein is part of the structural maintenance of chromosomes (SMC) 5/6 complex which plays a key role genome maintenance, facilitating chromosome segregation and suppressing mitotic recombination. A knockout of the orthologous mouse gene is lethal prior to embryonic day 10.5. Naturally occurring mutations in this gene, that abolish the SUMO ligase activity, are associated with primordial dwarfism and extreme insulin resistance. [provided by RefSeq, Mar 2017]
