Phosphate Assay Kit (Fluorometric) (BN00669)

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ELISA Kit Technical ManualMSDS

Phosphate Assay Kit (Fluorometric)

Inorganic phosphate (Pi) is one of the most important ions in biological systems. It functions in a variety of roles. One of its most important roles is as a molecular switch, turning enzyme activity on and off through the mediation of the various protein kinases and phosphatases in biological systems. A highly sensitive assay is desired to monitor Pi in a variety of samples. The newly designed and enhanced Phosphate Assay Kit provides a sensitive, easy and quick means of assessing Pi over a wide range of concentrations. In this assay, inorganic phosphate reacts with sucrose to produce glucose-1-phosphate in the presence of a proprietary enzyme. The glucose-1-phosphate is specifically oxidized to generate a product that reacts with the to generate fluorescence (Ex/Em = 535/587 nm). The kit can be used to detect Pi produced through reactions involving ATPases, GTPases, 5'-nucleotidase, protein phosphatases, acid and alkaline phosphatases, phosphorylase, etc. from a variety of samples. Unlike other commercially available assays, this assay is not affected by the presence of glucose in samples. This kit can determine phosphate concentrations between 2 µM and 10 µM, with a lower detection limit of approximately 100 pmol.

Figure 1: Phosphate standard curve made according to the protocol (n=3, error bars represent standard deviation).

Key Information Description

Product SKU



100 assays

Detection Method

Fluorescence (Ex/Em 535/587nm)

Species Reactivity



Measure Phosphate concentrations between 2 µM and 10 µM, with a lower detection limit of approximately 100 pmol.

Features and Benefits

  • Simple procedure; takes ~ 3 hours
  • Fast and convenient
  • The assay is sensitive and stable The TUNEL-based assay kit provides complete components including positive and negative control cells for convenient detection of DNA fragmentation in cultured cells and tissue sections.

Kit Components

  • Phosphate Assay Buffer(in DMSO)
  • Converter
  • Developer
  • Phosphate Substrate

Storage Conditions


Shipping Conditions

Gel Pack


For Research Use Only! Not For Use in Humans.

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Additional Information

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