Phosphoglucomutase Assay Kit (Fluorometric) (BN00983)

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BN00983
  • Phosphoglucomutase Assay Kit (Fluorometric) (BN00983)
  • Phosphoglucomutase Assay Kit (Fluorometric) (BN00983)
  • Phosphoglucomutase Assay Kit (Fluorometric) (BN00983)
€852

Description

ELISA Kit Technical ManualMSDS

Phosphoglucomutase Assay Kit (Fluorometric)

Phosphoglucomutase (PGM) enzyme plays a key role in carbohydrate metabolism and widely exists in all organisms. PGM interconverts Glucose-1-Phosphate (G1P) and Glucose-6-Phosphate (G6P) depending on the body requirement. When glycogen breaks down, G1P is generated and phosphoglucomutase converts G1P to G6P, which can go either to glycolytic pathway to generate ATP, or to pentose phosphate pathway to generate ribose and NADPH. On the other hand, when cells have extra energy, PGM converts G6P to G1P, which generates glycogen. In humans, phosphoglucomutase has 2 isoforms (PGM I and PGM II). PGM deficiency leads to glucose storage disease. Detection of abnormal phosphoglucomutase activity is crucial for diagnosis, prediction and treatment of the disease. In Assay Genie's phosphoglucomutase assay, PGM converts glucose-1-phosphate to glucose-6-phosphate; the glucose-6-phosphate is oxidized by glucose-6-phosphate dehydrogenase to form NADPH, which reduces to generate an intense fluorescence product (Ex/Em = 535/587 nm). This assay kit is simple, highly sensitive, and rapid and can detect as low as 20 of PGM activity.

Figure: (a).NADPH standard curve (b). Phosphoglucomutase activity in Jurkat cell lysate (0.4 μg), rat liver homogenate (0.2 μg), and Positive Control Phosphoglucomutase (c).specific activity in Jurkat cell lysate and rat liver homogenate Assays were performed following the kit protocol.

Key Information Description

Product SKU

BN00983

Size

100 assays

Detection Method

Fluorescence (Ex/Em = 535/587 nm)

Applications

  • Measurement of phosphoglucomutase activity in various tissues/cells.
  • Analysis of glucose metabolism and cell signaling in various cell types.
  • Screening of anti-diabetic drugs.

Features and Benefits

  • Rapid, Convenient and Reliable
  • The assay can detect as low as 20 of PGM activity

Kit Components

  • PGM Assay Buffer (in DMSO)
  • PGM Substrate
  • PGM Enzyme Mix
  • PGM Developer NADPH
  • Standard (200 nmol)
  • PGM Positive Control

Storage Conditions

-20°C

Shipping Conditions

Gel Pack

USAGE

For Research Use Only! Not For Use in Humans.

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