Protein Disulfide Isomerases (PDI) Inhibitor Screening Kit (Fluorometric) (BN01057)

(No reviews yet) Write a Review
SKU:
BN01057
  • Protein Disulfide Isomerases (PDI) Inhibitor Screening Kit (Fluorometric) (BN01057)
  • Protein Disulfide Isomerases (PDI) Inhibitor Screening Kit (Fluorometric) (BN01057)
€792

Description

ELISA Kit Technical ManualMSDS

Protein Disulfide Isomerases (PDI) Inhibitor Screening Kit (Fluorometric)

Protein Disulfide Isomerases (PDI, EC: 5.3.4.1) constitute a thio-disulfide oxidoreductase family. PDI is abundant in the lumen of endoplasmic reticulum (ER). PDI plays an essential role in catalyzing the rearrangement of S-S bonds in proteins and functions as a chaperon. Recent studies show that PDI activity is essential for cancer cell survival and proliferation, and targeting the PDI activity with its inhibitors abrogates survival responses to ER stress in cancer cells. Thus, identification and development of PDI inhibitors represents an important approach in cancer therapy. In Assay Genie's PDI Inhibitor Screening Kit, the PDI converts Insulin into its reduced form in the presence of Dithiothreitol (DTT). This reduced insulin binds to a fluorescent probe to generate an intensely fluorescent product (Ex/Em = 440/490 nm). In the presence of a PDI inhibitor, the reaction is impeded/abolished, resulting in a decrease or total loss of fluorescence. This assay kit can be used to screen/study/characterize the potential inhibitors of Protein Disulfide Isomerase. The assay is simple, high-throughput adaptable and can be performed within 60 min.

Figure: Inhibition of Protein Disulfide Isomerase activity by PDI Inhibitors (Bacitracin and Iodoacetamide). For Iodoacetamide, IC 50 = 17µM. Assay was performed following the kit protocol.

Key Information Description

Product SKU

BN01057

Size

100 assays

Detection Method

Fluorescence (Ex/Em = 440/490 nm)

Applications

Screening/characterizing/studying potential inhibitors of Protein Disulfide Isomerase (PDI)

Features and Benefits

  • Simple and High throughput adaptable
  • 60 minutes short protocol
  • Detect using a fluorometer or fluorescence microplate reader.

Kit Components

  • PDI Assay Buffer
  • PDI Substrate
  • PDI Probe (in DMSO) (20X)
  • DTT (100X)
  • PDI Enzyme
  • PDI Inhibitor Control (Iodoacetamide)

Storage Conditions

-20°C

Shipping Conditions

Gel Pack

USAGE

For Research Use Only! Not For Use in Humans.

View AllClose