Protein FITC Labeling Kit (BN01049)

SKU:
BN01049
€482

Description

ELISA Kit Technical ManualMSDS

Protein FITC Labeling Kit

Assay Genie's Protein-FITC Labeling Kit provides an easy way to label proteins with Fluorescein Isothiocyanate (FITC) in a user-friendly spin column format. FITC is an ideal dye for fluorescent labeling of proteins among the most commonly used fluorescent dyes for labeling proteins. Each spin column provided in the kit can be used to label up to 1 mg of the target protein. The kit provides all of the reagents necessary to perform five labeling reactions using up to 1 mg of protein per reaction. FITC-labeled protein has an excitation and emission wavelengths at 494 nm and 520 nm respectively, and can be directly used for downstream applications including ELISA, western blot, Immunohistochemistry, Immunofluorescence, and FACS analysis, etc.

Figure: SEC chromatogram of a BSA labeled with FITC using a Superdex 200 HR 10/30 column at 0.5 ml/min. in 50 mM Tris and 0.25 M NaCl pH 7.5. The absorbance was monitored at 280 nm (Blue line) and 494 nm (Red line). The overlapping peaks of 280 nm and 494 nm indicate successful labeling of the protein with FITC. In addition, the spin column format ensured that the purification of protein was fast and there was no unreacted FITC left after the protein was purified according to the kit protocol.

Key Information Description

Product SKU

BN01049

Size

For 5 x 1 mg of protein

Detection Method

Fluorescence (Ex/Em = 494/520 nm)

Applications

  • Labeled proteins can be used for ELISA, western blot, Immunohistochemistry, Immunofluorescence, and FACS analysis.
  • Easy to use Kit to label upto 5 mg of Protein with FITC for multiple applications such as ELISA, western blot, Immunohistochemistry, Immunofluorescence and FACS analysis

Features and Benefits

  • All necessary reagents included.
  • Fast and efficient labeling of proteins with FITC

Kit Components

  • EZLabel FITC
  • EZLabel Spin Column
  • EZLabel Quenching Buffer
  • EZLabel Elution Buffer

Storage Conditions

4°C

Shipping Conditions

Gel Pack

USAGE

For Research Use Only! Not For Use in Humans.

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Additional Information

Product type:
Molecular Tools
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