Recombinant Human 4BB/TNFRSF9 Protein (His Tag)(Active) (RPES1720)

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ELISA Kit Technical Manual

Recombinant Human 4BB/TNFRSF9 Protein (His Tag)(Active)

Background:Tumor necrosis factor receptor superfamily member 9(TNFRSF9), also known as CD137 and 4BB, is an inducible T cell surface protein belonging to the tumor necrosis factor receptor superfamily. It is a single-pass type I membrane protein which contains 4 TNFR-Cys repeats. The human and mouse proteins share 60% amino acid sequence identity. CD137 is expressed by mesenchymal cells, including endothelial cells, chondrocytes, and cells of the central nervous system. CD137 is also broadly expressed by cells of the human immune system, is broadly expressed by cells of the human immune system, including activated CD8+ and CD4+ T cells, activated natural killer (NK) cells, follicular dendritic cells (FDCs) and monocytes. CD137 has diverse roles in the immune response, the one key function is to promote the survival of both T cells and dendritic cells by binding the cognate ligand CD137L (4BBL).
Name:Recombinant Human 4BB/TNFRSF9 Protein (His Tag)(Active)
Assay Genie SKU:RPES1720
Synonyms:CD137; ILA; TNFRSF9; 4BB ligand receptor; CDw137; T-cell antigen 4BB homolog; T-cell antigen ILA
Expression host:Human Cells
Mol Mass:18.1 kDa
AP Mol Mass:28-35 kDa
Bio Activity:Immobilized Human 4BB-His at 10?g/ml(100 ?l/well) can bind Human 4BB-Fc(Cat: PKSH032024). The ED50 of Human 4BB-His is 0.4 ug/ml .
Purity:> 95 % as determined by reducing SDS-PAGE.
Endotoxin:< 1.0 EU per µg as determined by the LAL method.
Storage:Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8? for 2-7 days. Aliquots of reconstituted samples are stable at < -20'C for 3 months.
Shipping:This product is provided as lyophilized powder which is shipped with ice packs.
Formulation:Lyophilized from a 0.2 um filtered solution of PBS, pH7.4.
Reconstitution:Please refer to the printed manual for detailed information.
Application:Functional ELISA
UniProt Protein Function:TNFRSF9: Receptor for TNFSF9/4BBL. Possibly active during T cell activation.
UniProt Protein Details:

Protein type:Membrane protein, integral; Receptor, misc.

Chromosomal Location of Human Ortholog: 1p36

Cellular Component: extracellular space; integral to plasma membrane; external side of plasma membrane

Molecular Function:cytokine binding; receptor activity

Biological Process: negative regulation of cell proliferation; apoptosis

NCBI Summary:The protein encoded by this gene is a member of the TNF-receptor superfamily. This receptor contributes to the clonal expansion, survival, and development of T cells. It can also induce proliferation in peripheral monocytes, enhance T cell apoptosis induced by TCR/CD3 triggered activation, and regulate CD28 co-stimulation to promote Th1 cell responses. The expression of this receptor is induced by lymphocyte activation. TRAF adaptor proteins have been shown to bind to this receptor and transduce the signals leading to activation of NF-kappaB. [provided by RefSeq, Jul 2008]
UniProt Code:Q07011
NCBI GenInfo Identifier:728738
NCBI Gene ID:3604
NCBI Accession:Q07011.1
UniProt Related Accession:Q07011,AAB34924
Molecular Weight:27,899 Da
NCBI Full Name:Tumor necrosis factor receptor superfamily member 9
NCBI Synonym Full Names:tumor necrosis factor receptor superfamily, member 9
NCBI Official Symbol:TNFRSF9  
NCBI Official Synonym Symbols:ILA; 4BB; CD137; CDw137  
NCBI Protein Information:tumor necrosis factor receptor superfamily member 9; CD137 antigen; T cell antigen ILA; T-cell antigen ILA; 4BB ligand receptor; homolog of mouse 4BB; receptor protein 4BB; T-cell antigen 4BB homolog; induced by lymphocyte activation (ILA); interleukin-activated receptor, homolog of mouse Ly63
UniProt Protein Name:Tumor necrosis factor receptor superfamily member 9
UniProt Synonym Protein Names:4BB ligand receptor; CDw137; T-cell antigen 4BB homolog; T-cell antigen ILA; CD_antigen: CD137
UniProt Gene Name:TNFRSF9  
UniProt Entry Name:TNR9_HUMAN
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Additional Information

Product type:
Recombinant Protein
Host species:
Human Cells
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