High mobility group protein B1 is a member of the HMGB family consisting of three members; HMGB1; HMGB2 and HMGB3.It Contains 2 HMG box DNA-binding domains entitled box A and box B and It is a highly negative-charged C terminus. As a nuclear protein; HMGB1 stabilizes nucleosomes and allows bending of DNA that facilitates gene transcription which is essential for individual survival. Meanwhile; it is revealed that HMGB1 can also act as a cytokine extracellularlly and regulates monocyte; T cell; dendritic cell activities in inflammatory responses.
Human HMGB1 Recombinant Protein (RPES0717)
High Mobility Group Protein B1, High Mobility Group Protein 1, HMG-1, HMGB1, HMG1
<1.0 EU per µg as determined by the LAL method.
Recombinant Human High Mobility Group Protein B1 is produced by our Mammalian expression system and the target gene encoding Gly2-Glu215 is expressed with a 6His tag at the C-terminus.
> 95 % as determined by reducing SDS-PAGE.
AP Mol Mass:
Lyophilized from a 0.2 µm filtered solution of 20mM TrisHCl; 150mM NaCl; 1mM EDTA; pH 8.0.
This product is provided as lyophilized powder which is shipped with ice packs.
Stability and Storage:
Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.