Human SerpinE1/PAI Recombinant Protein (RPES4698)

SKU:
RPES4698
Product type:
Recombinant Protein
Host species:
Human Cells
Reactivity:
Human
$235

Description

ELISA Kit Technical Manual

Recombinant Human SerpinE1/PAI Protein (His Tag)

Background:Serpins are a group of proteins with similar structures that were first identified as a set of proteins able to inhibit proteases. They are the largest and most diverse family of serine protease inhibitors which are involved in a number of fundamental biological processes such as blood coagulation, complement activation, fibrinolysis, angiogenesis, inflammation and tumor suppression and are expressed in a cell-specific manner. Serpin E1 is a secreted protein which belongs to the Serpin family. Serpin E1 acts as 'bait' for tissue plasminogen activator, urokinase, and protein C. Its rapid interaction with TPA may function as a major control point in the regulation of fibrinolysis. Defects in SERPINE1 are characterized by abnormal bleeding due to Serpin E1 defect in the plasma. High concentrations of Serpin E1 have been associated with thrombophilia which is an autosomal dominant disorder in which affected individuals are prone to develop serious spontaneous thrombosis.
Name:Recombinant Human SerpinE1/PAI Protein (His Tag)
Size:10µg
Assay Genie SKU:RPES4698
Synonyms:Plasminogen Activator Inhibitor 1; PAI; PAI; Endothelial Plasminogen Activator Inhibitor; Serpin E1; SERPINE1; PAI1; PLANH1;SERPINE1
Species:Human
Expression host:Human Cells
Sequence:Val24-Pro402
Accession:P05121
Mol Mass:43.8 kDa
AP Mol Mass:48 kDa
Tag:C-6His
Purity:> 95 % as determined by reducing SDS-PAGE.
Endotoxin:< 1.0 EU per µg as determined by the LAL method.
Storage:Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8? for 2-7 days. Aliquots of reconstituted samples are stable at < -20'C for 3 months.
Shipping:This product is provided as lyophilized powder which is shipped with ice packs.
Formulation:Lyophilized from a 0.2 um filtered solution of 20mM HAc-NaAc, 150mM NaCl, pH 4.0.
Reconstitution:Please refer to the printed manual for detailed information.
UniProt Protein Function:SERPINE1: a secreted protein that acts as 'bait' for tissue plasminogen activator, urokinase, and protein C. Its rapid interaction with TPA may function as a major control point in the regulation of fibrinolysis. Belongs to the serpin family. Interacts with VTN. Binds LRP1B; binding is followed by internalization and degradation. Plasma levels of PAI and VCAM together may be useful in predicting post-operative recurrence in patients with colorectal cancer.
UniProt Protein Details:

Protein type:Secreted, signal peptide; Motility/polarity/chemotaxis; Secreted

Chromosomal Location of Human Ortholog: 7q22.1

Cellular Component: extracellular matrix; extracellular space; extracellular region; plasma membrane

Molecular Function:serine-type endopeptidase inhibitor activity; protein binding; protease binding; receptor binding

Biological Process: circadian rhythm; transcription initiation from RNA polymerase II promoter; platelet activation; extracellular matrix organization and biogenesis; positive regulation of blood coagulation; transcription, DNA-dependent; negative regulation of blood coagulation; negative regulation of smooth muscle cell migration; defense response to Gram-negative bacterium; positive regulation of receptor-mediated endocytosis; regulation of cell proliferation; positive regulation of interleukin-8 production; fibrinolysis; positive regulation of angiogenesis; platelet degranulation; negative regulation of fibrinolysis; transforming growth factor beta receptor signaling pathway; gene expression; positive regulation of transcription from RNA polymerase II promoter; regulation of receptor activity; angiogenesis; chronological cell aging; blood coagulation; negative regulation of cell adhesion mediated by integrin; negative regulation of cell migration; positive regulation of inflammatory response

Disease: Plasminogen Activator Inhibitor Deficiency

NCBI Summary:This gene encodes a member of the serine proteinase inhibitor (serpin) superfamily. This member is the principal inhibitor of tissue plasminogen activator (tPA) and urokinase (uPA), and hence is an inhibitor of fibrinolysis. Defects in this gene are the cause of plasminogen activator inhibitor deficiency (PAI deficiency), and high concentrations of the gene product are associated with thrombophilia. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2009]
UniProt Code:P05121
NCBI GenInfo Identifier:129576
NCBI Gene ID:5054
NCBI Accession:P05121.1
UniProt Secondary Accession:P05121,B7Z4S0, F8WD53,
UniProt Related Accession:P05121
Molecular Weight:43,404 Da
NCBI Full Name:Plasminogen activator inhibitor 1
NCBI Synonym Full Names:serpin peptidase inhibitor, clade E (nexin, plasminogen activator inhibitor type 1), member 1
NCBI Official Symbol:SERPINE1
NCBI Official Synonym Symbols:PAI; PAI1; PAI; PLANH1
NCBI Protein Information:plasminogen activator inhibitor 1; serpin E1; endothelial plasminogen activator inhibitor; serine (or cysteine) proteinase inhibitor, clade E (nexin, plasminogen activator inhibitor type 1), member 1
UniProt Protein Name:Plasminogen activator inhibitor 1
UniProt Synonym Protein Names:Endothelial plasminogen activator inhibitor; Serpin E1
UniProt Gene Name:SERPINE1
UniProt Entry Name:PAI1_HUMAN