2-Phosphoglycerate Colorimetric/Fluorometric Assay Kit (BN00991)

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BN00991
  • 2-Phosphoglycerate Colorimetric/Fluorometric Assay Kit (BN00991)
  • 2-Phosphoglycerate Colorimetric/Fluorometric Assay Kit (BN00991)
  • 2-Phosphoglycerate Colorimetric/Fluorometric Assay Kit (BN00991)
€702

Description

ELISA Kit Technical ManualMSDS

2-Phosphoglycerate Colorimetric/Fluorometric Assay

2-phosphoglycerate (2PG) is an important intermediate in the glycolysis pathway. 2-Phosphoglycerate is converted by enolase to phosphoenolpyruvate (PEP) which is a key step from glucose to pyruvate. Aberrant glycolytic metabolism is a highly studied and potentially critical mechanism for ATP generation in cancer cells (The Warburg effect). Measurement of intracellular 2PG levels is a useful tool for analyzing the glycolytic pathway and its relevance to cancer research. Assay Genie's 2-Phosphoglycerate Assay kit is a sensitive, fast and easy-to-use kit. In this assay, 2PG is converted by Enzyme Mix to PEP, which is further converted to pyruvate. The pyruvate is oxidized to generate color (OD 570 nm) and fluorescence (Ex/Em = 535/587 nm). The colored product or fluorescence intensity is proportional to 2PG level. This assay kit can detect 2PG level below 20 pmol and can be used for a variety of sample types.

(A)

(B)

(C)

Figure: 2PG Standard Curve: (A) Colorimetric and (B) Fluorometric. (C) Measurement of 2PG level in rat liver, heart and muscle lysate (200 µg protein each). Assays were performed according to kit protocol.

Key Information Description

Product SKU

BN00991

Size

100 assays

Detection Method

Absorbance (570 nm) or Fluorescence (Ex/Em 535/587 nm)

Applications

  • Measurement of 2-Phosphoglycerate in various tissues/cells
  • Analysis of carbohydrate metabolism and cell signaling
  • Cancer research

Features and Benefits

This assay kit can detect 2PG level below 20 pmol and can be used for a variety of sample types.

Kit Components

  • 2PG Assay Buffer
  • 2PG Probe (in DMSO)
  • 2PG Enzyme Mix (Lyophilized)
  • 2PG Converter (Lyophilized)
  • 2PG Developer (Lyophilized)
  • 2PG Standard (Lyophilized)

Storage Conditions

-20°C

Shipping Conditions

Gel Pack

USAGE

For Research Use Only! Not For Use in Humans.

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