Acidic Mammalian Chitinase Activity Kit (Fluorometric) (BN00745)

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BN00745
  • Acidic Mammalian Chitinase Activity Kit (Fluorometric) (BN00745)
  • Acidic Mammalian Chitinase Activity Kit (Fluorometric) (BN00745)
  • Acidic Mammalian Chitinase Activity Kit (Fluorometric) (BN00745)
€708

Description

ELISA Kit Technical ManualMSDS

Acidic Mammalian Chitinase Activity Kit (Fluorometric)

Acidic Mammalian Chitinase (AMCase) and Chitotriosidase-1(CHIT1) are the only two mammalian active chitinases that belong to the glycosyl hydrolase-18 family (EC 3.2.1.14). They are chitin-fragmenting hydrolases that hydrolyze the Beta-(1,4)-linkage between the adjacent N-acetyl glucosamine residues of chitin. In contrast to CHIT1, AMCase exhibits its optimum activity under acidic conditions and is relatively abundant in the gastrointestinal tract and lungs. AMCase is found highly expressed both in animal models used for the study of allergic inflammation and in lungs of human asthma. Recent data suggests a major biological function of AMCase in asthma and the changes in AMCase expression can be used to monitor Th-2/IL-13-mediated immune response and inflammation. Assay Genie's Acidic Mammalian Chitinase Activity Assay Kit utilizes the ability of AMCase to cleave a synthetic substrate to release the free fluorophore which can be easily quantified (Ex/Em= 320/445 nm) and it uses a specific Lysis Buffer which distinguishes AMCase activity from CHIT1 activity. This kit provides a simple, specific, ultra-sensitive assay that can detect as low as 0.5 mU/ml of AMCase activity in a variety of biological samples

Figure: (a) 4-Methylumbelliferon Standard Curve, results from multiple experiments. (b) Measurement of purified AMCase (2 ng) and CHIT1 (2 ng) activities. The kit can effectively discriminate AMCase activity from CHIT1 activity using our proprietary Lysis Buffer. (c) Measurement of AMCase activity in Rat Liver (20 µg protein) and Mouse Lung (2.5 µg protein). All assays were performed following kit protocol.

Key Information Description

Product SKU

BN00745

Size

100 assays

Detection Method

Fluorescence (Ex/Em 320/445 nm)

Species Reactivity

Mammalian

Applications

Measurement of Chitinase activity in various biological samples and purified/crude enzyme preparations

Features and Benefits

  • Simple, rapid & convenient assay to measure Acidic Mammalian Chitinase
  • Includes Positive Control (Acidic Mammalian Chitinase)

Kit Components

  • AMCase Assay Buffer
  • AMCase Lysis Buffer
  • AMCase Substrate (in DMSO)
  • Acidic Mammalian Chitinase (lyophilized)
  • 4-Methylumbelliferone Standard (5 mM)

Storage Conditions

-20°C

Shipping Conditions

Gel Pack

USAGE

For Research Use Only! Not For Use in Humans.

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