Actin Polymerization/Depolymerization Assay Kit (Fluorometric) (BN00695)

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BN00695
  • Actin Polymerization/Depolymerization Assay Kit (Fluorometric) (BN00695)
  • Actin Polymerization/Depolymerization Assay Kit (Fluorometric) (BN00695)
€840

Description

ELISA Kit Technical ManualMSDS

Actin Polymerization/Depolymerization Assay Kit (Fluorometric)

Actin, a highly conserved and abundant protein in eukaryotic cells, is one of the major components of cytoskeleton. It can be found as monomeric globular protein, called G actin or it can polymerize into filamentous actin, named F actin. Actin plays major roles in cell division, cell motility, cell signaling, organelle movement, etc. Mammals have 6 isoforms of Actin, which can be divided into 3 classes, Beta and Muscle Actin is class and all other non-muscle actins belong to Beta and -classes. Understanding the effect of different drugs, proteins, etc. on Actin Polymerization and Depolymerization is very important for understanding cellular machinery, more importantly because Cytoskeleton is a very important target for cancer therapy. Assay Genie's Actin Polymerization/Depolymerization Assay Kit can be used to study the effect of different compounds, proteins and tissue extracts on Actin polymerization and depolymerization. The kit utilizes a proprietary Pyrene-labeled Actin molecule that develops a higher fluorescent signal if it undergoes polymerization. The signal can be easily detected using a fluorescence microplate reader. The assay is simple, high- throughput compatible, and can be completed in less than three hours.

Figure: (a) Actin Polymerizarion: Actin Polymerization is induced by Buffer P. The process is inhibited by Latrunculin A (23 µM) (b) Actin Depolymerization: Polymerized Actin is depolymerized by Latrunculin A (23 µM). Assays were performed following the kit protocol.
Note: Latrunculin A is Actin polymerization inhibitor in vitro and in vivo by the formation of a 1:1 complex with monomeric G-actin.
Latrunculin A acts a depolymerization agent acting on Actin filaments (Factin).

Key Information Description

Product SKU

BN00695

Size

100 assays

Detection Method

  • Fluorescence (Ex/Em: 365/410 nm)
  • Study and quantitate the effect of different compounds, proteins and tissue extracts on Actin polymerization and/or depolymerization
  • Evaluation of critical concentrations of actin polymerization in different conditions

Species Reactivity

Fluorescence (Ex/Em: 365/410 nm)

Applications

Not Applicable

Features and Benefits

  • Study and quantitate the effect of different compounds, proteins and tissue extracts on Actin polymerization and/or depolymerization
  • Evaluation of critical concentrations of actin polymerization in different conditions

Kit Components

  • Buffer G
  • Buffer P (10X)
  • Labeled Rabbit Muscle Actin
  • ATP (100 mM)

Storage Conditions

-20°C

Shipping Conditions

Gel Pack

USAGE

For Research Use Only! Not For Use in Humans.

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