Adenylate Kinase (AK) Activity Assay Kit (Colorimetric/Fluorometric) (BN00614)

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SKU:
BN00614
  • Adenylate Kinase (AK) Activity Assay Kit (Colorimetric/Fluorometric) (BN00614)
  • Adenylate Kinase (AK) Activity Assay Kit (Colorimetric/Fluorometric) (BN00614)
  • Adenylate Kinase (AK) Activity Assay Kit (Colorimetric/Fluorometric) (BN00614)
  • Adenylate Kinase (AK) Activity Assay Kit (Colorimetric/Fluorometric) (BN00614)
€619

Description

ELISA Kit Technical ManualMSDS

Adenylate Kinase (AK) Activity Assay Kit (Colorimetric/Fluorometric)

Adenylate Kinase (AK) (EC 2.7.4.3) is an abundant enzyme involved in energy metabolism and homeostasis of cellular adenine nucleotide ratios in different intracellular compatments. The enzyme is found in the nucleus, cytosol, or mitochondria (intermembrane space or matrix) of various kinds of tissues. Adenylate kinase acts on two molecules of ADP to generate ATP and AMP. Nine isoforms of adenylate kinase have been identified. Erythrocyte adenylate kinase deficiency is associated with hemolytic anemia. Adenylate kinase also plays an important role in post-ischemic recovery and in apoptosis. Assay Genie's AK Activity Assay kit can kinetically measure Adenylate Kinase activity by detecting adenosine triphosphate (ATP) generated from adenosine diphosphate (ADP) as a substrate. ATP is detected via a multi-step reaction, resulting in the generation of an intermediate that reacts with the Adenylate Kinase Probe forming an end product that can be measured colorimetrically (OD 570 nm) or fluorometrically (Ex/Em = 535/587 nm).

Figure: (a) ATP Standard Curve (fluorometric), (b) ATP Standard Curve (colorimetric), (c) Fluorometric quantitation of Adenylate Kinase Activity in bovine heart mitochondrial lysate (50 ng) and Positive Control (4 µl); (d) Colorimetric quantitation of Adenylate Kinase Activity in bovine heart mitochondrial lysate (500 ng) and Positive Control (5 µl).

Key Information Description

Product SKU

BN00614

Size

100 assays

Detection Method

Absorbance (OD 570 nm) or Fluorescence (Ex/Em = 535/587 nm)

Applications

Detection of Adenylate Kinase activity

Features and Benefits

  • Simple, rapid & convenient
  • The kit includes all the reagents required to measure AK activity

Kit Components

  • AK Assay Buffer
  • AK Probe
  • ADP Substrate
  • AK Convertor
  • AK Developer
  • Positive Control (AK Enzyme)
  • ATP Standard (1 µmol)

Storage Conditions

-20°C

Shipping Conditions

Gel Pack

USAGE

For Research Use Only! Not For Use in Humans.

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