Alkaline Phosphatase Activity Fluorometric Assay Kit (BN00671)

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SKU:
BN00671
€461

Description

ELISA Kit Technical ManualMSDS

Alkaline Phosphatase Activity Fluorometric Assay Kit

Alkaline phosphatase (ALP) catalyzes the hydrolysis of phosphate esters in alkaline buffer and produces an organic radical and inorganic phosphate. The change in alkaline phosphatase level and activity is associated with a lot of diseases in the liver and bones. Alkaline phosphatase is also a popular enzyme conjugated to secondary antibody in ELISA. In Assay Genie's Alkaline Phosphatase Fluorimetric Assay Kit, ALP cleaves the phosphate group of the non-fluorescent 4-Methylumbelliferyl phosphate disodium salt (MUP) substrate resulting in an intense fluorescent signal (Ex/Em = 360nm/440nm). The kit is an ultra sensitive, simple, direct and HTS-ready assay designed to measure ALP activity in serum and bio-samples with detection sensitivity ~1 µU, more sensitive than colorimetric assays. The kit is suitable for both research and drug discovery.

Key Information Description

Product SKU

BN00671

Size

500 assays

Detection Method

Fluorescence (360/440)

Species Reactivity

Mammalian

Applications

The assay designed to measure ALP activity in serum and bio-samples with detection sensitivity ~1 µU

Features and Benefits

  • Simple procedure; takes less than 1 hour
  • Fast and convenient
  • The kit is an ultra-sensitive, direct and HTS-ready fluorometric assay designed to measure AP activity in serum and other bio-samples.

Kit Components

  • ALP Assay Buffer
  • MUP Substrate
  • ALP Enzyme
  • Stop Solution

Storage Conditions

-20°C

Shipping Conditions

Gel Pack

USAGE

For Research Use Only! Not For Use in Humans.

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