Alpha-Ketoglutarate Dehydrogenase Activity Colorimetric Assay Kit (BN00902)

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BN00902
  • Alpha-Ketoglutarate Dehydrogenase Activity Colorimetric Assay Kit (BN00902)
  • Alpha-Ketoglutarate Dehydrogenase Activity Colorimetric Assay Kit (BN00902)
  • Alpha-Ketoglutarate Dehydrogenase Activity Colorimetric Assay Kit (BN00902)
€912

Description

ELISA Kit Technical ManualMSDS

Alpha-Ketoglutarate Dehydrogenase Activity Colorimetric Assay Kit

Ketoglutarate Dehydrogenase (KGDH) (EC 1.2.4.2) is a key enzyme in the citric acid cycle. It forms an enzyme complex with dihydrolipoamide succinyl transferase (E2) and dihydrolipoamide dehydrogenase (E3). KGDH converts ketoglutarate into succinyl-CoA in the presence of NAD and CoA. It is highly regulated by intracellular ATP/ADP and NADH/NAD ratios and calcium. In humans, decreased KGDH activity can lead to neurodegenerative diseases such as Alzheimer's disease. Recent studies show that KGDH is a target of oxidative stress; reactive oxygen species (ROS) inhibit KGDH activity which diminishes its critical function and can cause a bioenergetic deficit. Assay Genie's KGDH assay kit provides a quick and easy way for monitoring KGDH activity in various samples. In the assay, KGDH converts ketoglutarate into an intermediate which reduces the probe to a colored product with strong absorbance at 450 nm. The assay is simple, sensitive and can detect ketoglutarate dehydrogenase activity lower than 0.1 mU in a variety of samples.

Figure: (a) NADH standard curve; (b) α-Ketoglutarate Dehydrogenase activity in rat heart (75 μg) and liver lysates (100 μg); (c) α-Ketoglutarate Dehydrogenase specific activity was calculated in rat heart lysate (75 μg), yeast mitochondria prepared from S.Cerevisiae (10 µg) and in rat liver lysate (100 μg). Assays were performed following the kit protocol.

Key Information Description

Product SKU

BN00902

Size

100 assays

Detection Methods

Absorbance (450 nm)

Applications

  • Measurement of Ketoglutarate dehydrogenase activity in various tissues/cells
  • Analysis of cell signaling pathways such as citrate acid cycle, lysine degradation or tryptophan metabolism in various cell types

Features and Benefits

  • Simple, rapid & convenient
  • Can measure ketoglutarate dehydrogenase activity lower than 0.1 mU in a variety of samples

Kit Components

  • KGDH Assay Buffer
  • KGDH Substrate (Lyophilized)
  • KGDH Developer (Lyophilized)
  • NADH Standard (Lyophilized)
  • KGDH Positive Control

Storage Conditions

-20°C

Shipping Conditions

Gel Pack

USAGE

For Research Use Only! Not For Use in Humans.

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