Alpha Ketoglutarate Dehydrogenase Activity Assay (BN00902)

SKU:
BN00902
  • Metabolism Assays Alpha Ketoglutarate Dehydrogenase Activity Assay BN00902
  • Metabolism Assays Alpha Ketoglutarate Dehydrogenase Activity Assay BN00902
  • Metabolism Assays Alpha Ketoglutarate Dehydrogenase Activity Assay BN00902
€912

Description

Technical Manual MSDS

Alpha Ketoglutarate Dehydrogenase Activity Assay Kit

Assay Genie’s Alpha Ketoglutarate Dehydrogenase Activity assay kit provides a quick and easy way for monitoring Alpha Ketoglutarate Dehydrogenase (alpha KGDH) activity. In the assay, alpha KGDH converts alpha ketoglutarate into an intermediate which reduces the probe to a colored product with strong absorbance at 450 nm. The assay is simple, sensitive and can detect alpha ketoglutarate dehydrogenase activity lower than 0.1 mU in a variety of samples.

Alpha KGDH Assay Kit Data

Assay Information Description
Product Name: Alpha Ketoglutarate Dehydrogenase Activity Assay Kit
Product SKU: BN00902
Size: 100 assays
Application:
  • Measurement of Ketoglutarate dehydrogenase activity in various tissues/cells
  • Analysis of cell signaling pathways such as citrate acid cycle, lysine degradation or tryptophan metabolism
Detection Method: Colorimetric (Absorbance 450 nm)
Sensitivity: ≤0.1 mU
Sample Type:
  • Animal tissues: liver, heart, muscle, etc
  • Purified mitochondria
  • Cell culture: Adherent or suspension cells
Storage: -20°C
Shipping Conditions: Gel Pack
Usage: For research use only. Not for use in humans.

Alpha KGDH Assay Kit Components

Component Quantity
KGDH Assay Buffer 25 ml
KGDH Substrate (Lyophilized) 1 vial
KGDH Developer (Lyophilized) 1 vial
NADH Standard (Lyophilized) 1 vial
KGDH Positive Control 50µl

Product Images

Figure: (a) NADH standard curve; (b) Alpha Ketoglutarate Dehydrogenase activity in rat heart (75 μg) and liver lysates (100 μg); (c) Alpha Ketoglutarate Dehydrogenase specific activity was calculated in rat heart lysate (75 μg), yeast mitochondria prepared from S.Cerevisiae (10 µg) and in rat liver lysate (100 μg). Assays were performed following the kit protocol.

Alpha Ketoglutarate Dehydrogenase Background

Alpha Ketoglutarate Dehydrogenase (alpha KGDH) (EC 1.2.4.2) is a key enzyme in the citric acid cycle. It forms an enzyme complex with dihydrolipoamide succinyl transferase (E2) and dihydrolipoamide dehydrogenase (E3). Alpha KGDH converts alpha ketoglutarate into succinyl-CoA in the presence of NAD and CoA. It is highly regulated by intracellular ATP/ADP and NADH/NAD ratios and calcium. In humans, decreased KGDH activity can lead to neurodegenerative diseases such as Alzheimer’s disease. Recent studies show that alpha KGDH is a target of oxidative stress; reactive oxygen species (ROS) inhibit KGDH activity which diminishes its critical function and can cause a bioenergetic deficit.

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Additional Information

Product type:
Cellular Assay
Detection Method:
Colorimetric
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