Alpha Ketoglutarate Dehydrogenase Activity Assay Kit
Assay Genie’s Alpha Ketoglutarate Dehydrogenase Activity assay kit provides a quick and easy way for monitoring Alpha Ketoglutarate Dehydrogenase (alpha KGDH) activity. In the assay, alpha KGDH converts alpha ketoglutarate into an intermediate which reduces the probe to a colored product with strong absorbance at 450 nm. The assay is simple, sensitive and can detect alpha ketoglutarate dehydrogenase activity lower than 0.1 mU in a variety of samples.
Alpha KGDH Assay Kit Data
|Product Name:||Alpha Ketoglutarate Dehydrogenase Activity Assay Kit|
|Detection Method:||Colorimetric (Absorbance 450 nm)|
|Sample Type:|| |
|Shipping Conditions:||Gel Pack|
|Usage:||For research use only. Not for use in humans.|
Alpha KGDH Assay Kit Components
|KGDH Assay Buffer||25 ml|
|KGDH Substrate (Lyophilized)||1 vial|
|KGDH Developer (Lyophilized)||1 vial|
|NADH Standard (Lyophilized)||1 vial|
|KGDH Positive Control||50µl|
Figure: (a) NADH standard curve; (b) Alpha Ketoglutarate Dehydrogenase activity in rat heart (75 μg) and liver lysates (100 μg); (c) Alpha Ketoglutarate Dehydrogenase specific activity was calculated in rat heart lysate (75 μg), yeast mitochondria prepared from S.Cerevisiae (10 µg) and in rat liver lysate (100 μg). Assays were performed following the kit protocol.
Alpha Ketoglutarate Dehydrogenase Background
Alpha Ketoglutarate Dehydrogenase (alpha KGDH) (EC 184.108.40.206) is a key enzyme in the citric acid cycle. It forms an enzyme complex with dihydrolipoamide succinyl transferase (E2) and dihydrolipoamide dehydrogenase (E3). Alpha KGDH converts alpha ketoglutarate into succinyl-CoA in the presence of NAD and CoA. It is highly regulated by intracellular ATP/ADP and NADH/NAD ratios and calcium. In humans, decreased KGDH activity can lead to neurodegenerative diseases such as Alzheimer’s disease. Recent studies show that alpha KGDH is a target of oxidative stress; reactive oxygen species (ROS) inhibit KGDH activity which diminishes its critical function and can cause a bioenergetic deficit.