Ammonia Colorimetric Assay Kit (BN00632)

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BN00632
  • Ammonia Colorimetric Assay Kit (BN00632)
  • Ammonia Colorimetric Assay Kit (BN00632)
  • Ammonia Colorimetric Assay Kit (BN00632)
€619

Description

ELISA Kit Technical ManualMSDS

Ammonia Colorimetric Assay Kit

Ammonia is an important source of nitrogen for living systems. Nitrogen is required for the synthesis of amino acids, which are the building blocks of protein. Ammonia is a metabolic product which is created through amino acid deamination. It plays an important role in both normal and abnormal animal physiology such as acid/base balance. Assay Genie provides a rapid, simple, sensitive, and reliable assay suitable for research and high throughput assay of Ammonia. In the assay, Ammonia is converted to a product that reacts with the GenieRed probe to generate color (ax = 570 nm) which can be easily quantified by plate reader. The kit can detect 1 nmol (~20 µM) of ammonia, much more sensitive than measuring NADPH based ammonia assay.

Figure. (a) Ammonia Standard Curve (b) Ammonia concentration in human urine (25 µl, 100 times diluted) (c) and HeLa cell lysate (3 µl, 10.8 mg/ml). Assay was performed according to the kit protocol.

Key Information Description

Product SKU

BN00632

Size

100 assays

Detection Method

Absorbance (570 nm)

Species Reactivity

Mammalian

Applications

The kit can detect 1 nmol (~20 µM) of ammonia, much more sensitive than measuring NADPH based ammonia assay.

Features and Benefits

  • Simple procedure; takes ~ 1 hour
  • Fast and convenient
  • The assay is sensitive, stable and high-throughput adaptable

Kit Components

  • Ammonia Assay Buffer
  • GenieRed Probe in DMSO
  • Enzyme Mix (lyophilized)
  • Developer
  • Converting Enzyme (Lyophilized)
  • NHl Standard (100 mM)

Storage Conditions

-20°C

Shipping Conditions

Gel Pack

USAGE

For Research Use Only! Not For Use in Humans.

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