Ammonia Colorimetric Assay Kit II (BN00704)

SKU:
BN00704
€619

Description

ELISA Kit Technical ManualMSDS

Ammonia Colorimetric Assay Kit II

Ammonia is an important source of nitrogen for living systems. Nitrogen is required for the synthesis of amino acids, which are the building blocks of protein. Ammonia is a metabolic product which is created through amino acid deamination. It plays an important role in both normal and abnormal animal physiology such as acid/base balance. Assay Genie provides a rapid, simple, sensitive, and reliable assay suitable for research and high throughput assay of Ammonia. In this non-enzymatic assay, ammonia is used to form indophenol, a highly colored product easily quantifiable by colorimetry (OD670 nm) using a plate reader. The kit can detect less than 1 nmol (~10 ) ammonia using either a 96 well or 384 well microwell plate.

Figure: Standard Curve generated in standard flat-bottomed 96 well plates.

Key Information Description

Product SKU

BN00704

Size

100 assays

Detection Method

Absorbance (670 nm)

Species Reactivity

Mammalian

Applications

The kit can detect less than 1 nmol (~10 ) ammonia using either a 96 well or 384 well microwell plate.

Features and Benefits

  • Simple procedure; takes ~ 3 hours
  • Fast and convenient
  • The assay is sensitive and stable
  • The TUNEL-based assay kit provides complete components including positive and negative control cells for convenient detection of DNA fragmentation in cultured cells and tissue sections.

Kit Components

  • Ammonia Reagent 1
  • Ammonia Reagent 2
  • Ammonium Chloride Standard (100 mM)

Storage Conditions

4°C

Shipping Conditions

Gel Pack

USAGE

For Research Use Only! Not For Use in Humans.

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Additional Information

Product type:
Cellular Assay
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