Angiotensin I Converting Enzyme (ACE1) Inhibitor Screening Kit (BN00501)
Write a Review
×Angiotensin I Converting Enzyme (ACE1) Inhibitor Screening Kit (BN00501)
- SKU:
- BN00501
Description
Angiotensin I Converting Enzyme (ACE1) Inhibitor Screening Kit
Angiotensin I converting enzyme (ACE1, EC: 3.4.15.1), a dipeptidyl carboxypeptidase, is part of the renin-angiotensin system (RAS) that controls regulation of blood pressure by cleaving the C-terminal dipeptides of angiotensin I and bradykinin. It is found on the luminal surface of vascular endothelial cells, especially in pulmonary tissues. In addition, elevated levels of ACE1 are found in sarcoidosis, leprosy, hyperthyroidism, acute hepatitis, primary biliary cirrhosis, diabetes mellitus, multiple myeloma, osteoarthritis, amyloidosis, Gaucher disease, pneumoconiosis, histoplasmosis and miliary tuberculosis. Assay Genie's ACE1 Inhibitor Screening Kit can be used to screen for potent inhibitors of ACE1 activity to regulate hypertension. It utilizes the ability of an active ACE1 to cleave a synthetic o-aminobenzoyl peptide (Abz-based peptide substrate to release a free fluorophore). The released Abz can be easily quantified using a fluorescence microplate reader. In the presence of an ACE1 specific inhibitor, the enzyme loses its peptidase activity which results in decrease of fluorescence intensity. This assay kit is simple and can be used to identify and characterize ACE1 inhibitors in a high-throughput format
Figure: Inhibition of ACE1 activity by ACE1 Inhibitor Captopril (IC 50 = 1.46 nM (n = 3)). Assay was performed following the kit protocol.
Key Information | Description |
Product SKU | BN00501 |
Size | 100 assays |
Detection Method | Fluorometric (Ex/Em = 330/430 nm) |
Applications | Screening/studying/characterizing inhibitors of ACE1 |
Features and Benefits |
|
Kit Components |
|
Storage Conditions | -20°C |
Shipping Conditions | Gel Pack |
USAGE | For Research Use Only! Not For Use in Humans. |
Related Products


Angiotensin I Converting Enzyme Activity (ACE1) Assay Kit (Fluorometric) (BN00500)


Lysozyme Inhibitor Screening Kit (BN00505)


Angiotensin II Converting Enzyme (ACE2) Activity Assay Kit (Fluorometric) (BN01071)

Angiotensin II Converting Enzyme (ACE2) Inhibitor Screening Kit (BN00569)

G6PDH Inhibitor Screening Kit (BA0194)
Customers Also Viewed


Aminopeptidase N (APN/CD13) Activity Assay Kit (Fluorometric) (BN00755)


Angiotensin I Converting Enzyme Activity (ACE1) Assay Kit (Fluorometric) (BN00500)

5'-Nucleotidase Activity Assay Kit (Colorimetric) (BN01150)


Lactate Dehydrogenase Activity Colorimetric Assay Kit (384-well) (BN01114)


Human Inosine-5-Monophosphate Dehydrogenase (IMPDH) Inhibitor Screening Assay Kit (BN00728)


Dihydrofolate Reductase Inhibitor Screening Kit (Colorimetric) (BN00512)

LDH Cytotoxicity Assay Kit (BA0001)


3D Cell Culture HTS Cell Viability Assay Kit (BN01109)


Lysyl Oxidase Activity Assay Kit (Fluorometric) (BN01101)

