ACE1 Activity Assay Kit
Assay Genie's ACE1 Activity Assay Kit utilizes an active ACE1 to cleave a synthetic o-aminobenzoyl peptide (Abz-based peptide) substrate to release a fluorophore. The released Abz can be easily quantified using a fluorescence microplate reader. This assay kit is simple, rapid and can detect ACE activity as low as 10 mU in biological samples.
|Product Name:||ACE1 Activity Assay|
|Detection Method:||Fluorometric (Ex/Em = 330/430 nm)|
|Sample Type:|| |
|Assay Time:||1-2 hours|
|Storage:||Store the kit at -20°C, protect from light|
|Shipping Conditions:||Gel pack|
|Usage:||For research use only. Not for use in humans.|
ACE1 Activity Assay Kit Components
|ACE1 Assay Buffer||20 ml|
|ACE1 Lysis Buffer||40 ml|
|ACE1 Dilution Buffer||1 ml|
|ACE1 Positive control||5 µl|
|ACE1 Substrate||300 µl|
|Abz-Standard (1 mM)||100 µl|
Figure: (a) Abz-Standard Curve (0-1000 pmol), error bars indicate SD (n=3). (b) Kinetic activity curves using different amounts of ACE1 Positive Control in the assay. (c) ACE1 activity was measured for different types of rat tissue samples (lung, heart and kidney; 0.6 µg each). Assays were performed following the kit protocol.
Angiotensin I converting enzyme (ACE1, EC: 188.8.131.52), a dipeptidyl carboxypeptidase, is part of the renin-angiotensin system (RAS) that controls regulation of blood pressure by cleaving the C-terminal dipeptides of angiotensin I and bradykinin. It is found on the luminal surface of vascular endothelial cells, especially in pulmonary tissues. Elevated levels of ACE1 are found in patients suffering from sarcoidosis, leprosy, hyperthyroidism, acute hepatitis, primary biliary cirrhosis, diabetes mellitus, multiple myeloma, osteoarthritis, amyloidosis, Gaucher's disease, pneumoconiosis, histoplasmosis and miliary tuberculosis.