ACE1 Activity Assay Kit (BN00500)

SKU:
BN00500
  • Cell Biology Assays ACE1 Activity Assay Kit BN00500
  • Cell Biology Assays ACE1 Activity Assay Kit BN00500
  • Cell Biology Assays ACE1 Activity Assay Kit BN00500
€655

Description

Technical Manual MSDS

ACE1 Activity Assay Kit

Assay Genie's ACE1 Activity Assay Kit utilizes an active ACE1 to cleave a synthetic o-aminobenzoyl peptide (Abz-based peptide) substrate to release a fluorophore. The released Abz can be easily quantified using a fluorescence microplate reader. This assay kit is simple, rapid and can detect ACE activity as low as 10 mU in biological samples.

Assay Information Description
Product Name: ACE1 Activity Assay
Product SKU: BN00500
Size: 100 assays
Sensitivity: 10 mU
Application:
  • Determination of enzymatic activity of purified ACE1
  • Detection of ACE1 activity in tissue/cell lysates
Detection Method: Fluorometric (Ex/Em = 330/430 nm)
Reactivity: Mammalian
Sample Type:
  • Animal tissues: Lung, heart, kidney
  • Serum, plasma
Assay Time: 1-2 hours
Storage: Store the kit at -20°C, protect from light
Shipping Conditions: Gel pack
Usage: For research use only. Not for use in humans.

ACE1 Activity Assay Kit Components

Component Quantity
ACE1 Assay Buffer 20 ml
ACE1 Lysis Buffer 40 ml
ACE1 Dilution Buffer 1 ml
ACE1 Positive control 5 µl
ACE1 Substrate 300 µl
Abz-Standard (1 mM) 100 µl

Product Images

Figure: (a) Abz-Standard Curve (0-1000 pmol), error bars indicate SD (n=3). (b) Kinetic activity curves using different amounts of ACE1 Positive Control in the assay. (c) ACE1 activity was measured for different types of rat tissue samples (lung, heart and kidney; 0.6 µg each). Assays were performed following the kit protocol.

ACE1 Background

Angiotensin I converting enzyme (ACE1, EC: 3.4.15.1), a dipeptidyl carboxypeptidase, is part of the renin-angiotensin system (RAS) that controls regulation of blood pressure by cleaving the C-terminal dipeptides of angiotensin I and bradykinin. It is found on the luminal surface of vascular endothelial cells, especially in pulmonary tissues. Elevated levels of ACE1 are found in patients suffering from sarcoidosis, leprosy, hyperthyroidism, acute hepatitis, primary biliary cirrhosis, diabetes mellitus, multiple myeloma, osteoarthritis, amyloidosis, Gaucher's disease, pneumoconiosis, histoplasmosis and miliary tuberculosis.

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Additional Information

Product type:
Cellular Assay
Detection Method:
Fluorometric
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