Angiotensin II Converting Enzyme (ACE2) Activity Assay Kit (Fluorometric) (BN01071)

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BN01071
  • Angiotensin II Converting Enzyme (ACE2) Activity Assay Kit (Fluorometric) (BN01071)
  • Angiotensin II Converting Enzyme (ACE2) Activity Assay Kit (Fluorometric) (BN01071)
  • Angiotensin II Converting Enzyme (ACE2) Activity Assay Kit (Fluorometric) (BN01071)
  • Angiotensin II Converting Enzyme (ACE2) Activity Assay Kit (Fluorometric) (BN01071)
$621.18

Description

ELISA Kit Technical ManualMSDS

Angiotensin II Converting Enzyme (ACE2) Activity Assay Kit (Fluorometric)

Angiotensin II converting enzyme (ACE2, EC 3.4.17.23), a zinc-based metalloprotease, is part of the renin-angiotensin system (RAS) that controls the regulation of blood pressure by cleaving the C-terminal dipeptide of Angiotensin II to convert it into Angiotensin 1-7. ACE2 is a receptor of human coronaviruses, such as SARS and HCoV-NL63. It is expressed on the vascular endothelial cells of lung, kidney and heart. ACE2 is a potential therapeutic target for cardiovascular and coronavirus-induced diseases. Assay Genie's ACE2 Activity Assay Kit kit will help the research progress in this field. This kit utilizes the ability of an active ACE2 to cleave a synthetic MCA based peptide substrate to release a free fluorophore. The released MCA can be easily quantified using a fluorescence microplate reader. We also provide an ACE2 specific inhibitor that can differentiate the ACE2 activity from other proteolytic activity.The kit can detect as low as 0.4 mU. Our assay kit is simple and can be used in a high-throughput format.

Figure: (a) MCA -Standard Curve (0-300 pmol), error bars indicate SD (n=3). (b) Kinetic activity curves using different amounts of ACE2 Positive Control in the assay. (c) ACE2 activity was measured for different types of rat tissue samples (total protein in lung and kidney; 17 µg and 23 µg respectively), and human kidney tissue sample (10 µg total protein) in presence (+ Inhibitor) and absence (-Inhibitor) of ACE2 Inhibitor. (d) Spiked ACE2 activity and inhibition measured in HEK293 cell lysate (total protein: 37 µg). Assays were performed following the kit protocol

Key Information Description

Product SKU

BN01071

Size

100 assays

Detection Method

Fluorometric (Ex/Em = 320/420 nm)

Species Reactivity

Mammalian

Applications

Detection of ACE2 activity in tissue/cell lysates and enzyme preparations

Features and Benefits

  • Simple one-step reaction
  • Takes only 1-2 hrs
  • Non-radiometric fluorescent detection HTP adaptable

Kit Components

  • ACE2 Assay Buffer
  • ACE2 Dilution Buffer
  • ACE2 Lysis Buffer
  • ACE2 Positive Control
  • ACE2 Substrate
  • ACE2 Inhibitor (22 mM)
  • MCA-Standard (1 mM)

Storage Conditions

-20°C

Shipping Conditions

Gel Pack

USAGE

For Research Use Only! Not For Use in Humans.

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