Annexin V-EGFP Apoptosis Kit (BN00010)

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BN00010
€577 - €1,027

Description

ELISA Kit Technical ManualMSDS

Annexin V-EGFP Apoptosis Kit

The Annexin V-EGFP Apoptosis Detection Kit is based on the observation that soon after initiating apoptosis, most cell types translocate the membrane phospholipid phosphatidylserine (PS) from the inner face of the plasma membrane to the cell surface. Once on the cell surface, PS can be easily detected by staining with an enhanced green fluorescent protein (EGFP) fusion of annexin V, a protein that has a strong natural affinity for PS. The one-step staining procedure takes only 10 minutes. In addition, the assay can be directly performed on live cells. Detection can be analyzed by flow cytometry or by fluorescence microscopy with a FITC filter. EGFP is brighter and more photo-stable than other fluorescent reagents. The kit can differentiate apoptosis vs necrosis when performing both annexin V-EGFP and PI staining.

Figure: Detection of Apoptosis with Annexin V-EGFP. Apoptosis was induced in Jurkat cells by camptothecin (2µM) for various times as indicated. Cells were collected and incubated with Annexin V-EGFP for 5 minutes according to the kit instructions. Results were analyzed by flow cytometry.

Key Information Description

Detection Method

Flow cytometry (Ex = 488 nm; Em = 530 nm) and fluorescence microscopy

Size Options

  • 25 Assays
  • 100 Assays
  • 400 Assays

Species Reactivity

Mammalian

Application

Mammalian

Features and Benefits

  • Simple one step staining procedure in 10 minutes
  • Fast and convenient
  • EGFP is a bright and photo-stable green fluorescent protein and the kit can differentiate apoptosis vs necrosis when performing both annexin V-EGFP and PI staining

Kit Components

  • Annexin V-EGFP
  • 1X Binding Buffer
  • Propidium Iodide (PI)

Storage Conditions

4°C

Shipping Conditions

Gel Pack

USAGE

For Research Use Only! Not For Use in Humans.

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