Annexin V-FITC Apoptosis Kit Plus (BN00473)

SKU:
BN00473
Product type:
Cellular Assay
€577 - €1,016

Description

ELISA Kit Technical ManualMSDS

Annexin V-FITC Apoptosis Kit Plus

The Annexin V-FITC Apoptosis Detection Kit Plus is based on the observation that soon after initiating apoptosis, most cell types translocate the membrane phospholipid phosphatidylserine (PS) from the inner face of the plasma membrane to the cell surface. Once on the cell surface, PS can easily be detected by staining with a fluorescent conjugate of Annexin V, a protein that has a strong natural affinity for PS. The one-step staining procedure takes only 10 minutes. In addition, the assay can be directly performed on live cells, without the need of fixation. The Annexin V-FITC Apoptosis Detection Kit Plus includes annexin V-FITC, SYTOX green dye, and binding buffer. The SYTOX green dye is impermeant to live cells and apoptotic cells, but stains necrotic cells with intense green fluorescence by binding to cellular nucleic acids. After staining a cell population with annexin V-FITC and SYTOX Green dye in the provided binding buffer, apoptotic cells show green fluorescence, dead cells show a higher level of green fluorescence andLive cells show little or no fluorescence. These populations can easily be distinguished using a flow cytometry with the 488 nm line of an argon-ion laser for excitation. Both annexin V-FITC and SYTOX Green dye emit green fluorescence that can be detected in the FL1 channel, freeing the other channels for the addition of other probes in multi-color labeling experiments.

Key Information Description

Detection Method

Flow cytometry (Ex = 488 nm; Em = 530 nm).

Size Options

  • 25 Assays
  • 100 Assays
  • 400 Assays

Species Reactivity

Mammalian

Applications

Annexin V-FITC kit includes Annexin V-FITC for detecting apoptosis and also propidium iodide (PI) for detecting necrosis.

Features and Benefits

  • Simple one-step procedure; takes only 10 minutes
  • Fast and convenient
  • The Annexin VFITC Plus kit includes sytox green dye (instead of PI) which stains necrotic cells with a higher level of green fluorescence than FITC does

Kit Components

  • Annexin V-FITC
  • SYTOX Green Dye
  • Binding Buffer

Storage Conditions

4°C

Shipping Conditions

Gel Pack

USAGE

For Research Use Only! Not For Use in Humans.