The Huwe1 Polyclonal Antibody (CAB20708) is a valuable tool for researchers studying Huwe1, a protein involved in the regulation of various cellular processes, including cell cycle progression and DNA damage response. This antibody, produced in rabbits, is highly specific to human samples and has been validated for use in Western blotting applications. By binding to the Huwe1 protein, this antibody allows for the detection and analysis of Huwe1 expression in different cell types, making it suitable for studies in cancer biology and molecular biology.
Huwe1, also known as ARF-BP1, is a HECT domain-containing E3 ubiquitin ligase that plays a key role in the regulation of protein turnover and stability. Dysregulation of Huwe1 has been implicated in various diseases, including cancer and neurodegenerative disorders, making it a potential target for therapeutic intervention. Research on Huwe1 is essential for understanding its functions and developing strategies for targeted therapy in diseases associated with its aberrant activity.
Product Name:
HUWE1 Polyclonal Antibody
SKU:
CAB20708
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Rat
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 1-100 of human HUWE1 (NP_113584.3).
This gene encodes a protein containing a C-terminal HECT (E6AP type E3 ubiquitin protein ligase) domain that functions as an E3 ubiquitin ligase. The encoded protein is required for the ubiquitination and subsequent degradation of the anti-apoptotic protein Mcl1 (myeloid cell leukemia sequence 1 (BCL2-related)). This protein also ubiquitinates the p53 tumor suppressor, core histones, and DNA polymerase beta. Mutations in this gene are associated with Turner type X-linked syndromic cognitive disability.
Purification Method:
Affinity purification
Gene ID:
10075
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,50% glycerol,pH7.3.
Western blot analysis of various lysates using HUWE1 Rabbit pAb (CAB20708) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 180s.
