The SRD5A2 Polyclonal Antibody (CAB19762) is a valuable tool for researchers studying SRD5A2, an enzyme involved in the biosynthesis of dihydrotestosterone (DHT) from testosterone. This antibody, raised in rabbits, is highly specific to human samples and has been validated for use in Western blot applications. By targeting the SRD5A2 protein, researchers can accurately detect and analyze its expression in various cell types, making it an essential component for studies in hormonal regulation, prostate cancer, and other androgen-related disorders.
SRD5A2, also known as 5-alpha-reductase type 2, plays a crucial role in the conversion of testosterone into the more potent androgen DHT, which is implicated in various physiological processes and pathologies. Understanding the function and regulation of SRD5A2 is essential for developing therapies targeting androgen signaling pathways in conditions like prostate cancer and benign prostate hyperplasia. The SRD5A2 Polyclonal Antibody provides researchers with a reliable tool to further explore the role of this enzyme in health and disease.
Product Name:
SRD5A2 Rabbit Monoclonal Antibody
SKU:
CAB19762
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 1-100 of human SRD5A2 (P31213).
This gene encodes a microsomal protein expressed at high levels in androgen-sensitive tissues such as the prostate. The encoded protein is active at acidic pH and is sensitive to the 4-azasteroid inhibitor finasteride. Deficiencies in this gene can result in male pseudohermaphroditism, specifically pseudovaginal perineoscrotal hypospadias (PPSH).
Purification Method:
Affinity purification
Gene ID:
6716
Clone Number:
ARC2287
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,0.05% BSA,50% glycerol,pH7.3.
Western blot analysis of various lysates using SRD5A2 Rabbit mAb (CAB19762) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 180s.
