Ascorbic Acid Colorimetric Assay Kit II (FRASC) (BN00895)

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SKU:
BN00895
€619

Description

ELISA Kit Technical ManualMSDS

Ascorbic Acid Colorimetric Assay Kit II (FRASC)

Ascorbic Acid (Vitamin C) plays an important role in many biological processes. It is a potent anti-oxidant, anti-inflammatory, anti-viral agent and an immune stimulant and is present in a wide variety of biological specimens. Due to the presence of a variety of other antioxidants in biological samples such as serum, most ascorbic acid assays show strong interference. Assay Genie's FRASC Assay Kit provides a rapid, simple, and sensitive means of detecting ascorbic acid in biological samples such as serum and other body fluids, tissue and cell extracts, growth media and food products. In this assay, Fe is reduced to Fe²by any antioxidants present. The ferrous iron is chelated with a colorimetric probe to produce a product with a strong absorbance band which can be monitored between 545-600 nm. The addition of ascorbate oxidase to parallel samples removes any ascorbate present leaving a background value which is subtracted from the total to give ascorbate content. The assay can detect 0.2 to 20 nmol of ascorbic acid in various samples.

Figure: Ascorbic Acid Colorimetric Standard Curve obtained using kit protocol.

Key Information Description

Product SKU

BN00895

Size

100 assays

Detection Method

Absorbance (545-600 nm)

Species Reactivity

Mammalian

Applications

The assay can detect 0.2 to 20 nmol of ascorbic acid in various samples.

Features and Benefits

  • Simple procedure; takes ~ 30 minutes to complete the assay
  • Fast and convenient

Kit Components

  • FRASC Buffer
  • Ascorbic Acid Probe
  • FeCl3 solution
  • Ascorbate Oxidase (lyophilized)
  • Ascorbic Acid Standard (20 µmole)

Storage Conditions

-20°C

Shipping Conditions

Gel Pack

USAGE

For Research Use Only! Not For Use in Humans.

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