Aspartate Aminotransferase (AST or SGOT) Activity Colorimetric Assay Kit (BN00966)

SKU:
BN00966
  • Metabolism Assays Aspartate Aminotransferase AST or SGOT Activity Colorimetric Assay Kit BN00966
  • Metabolism Assays Aspartate Aminotransferase AST or SGOT Activity Colorimetric Assay Kit BN00966
€607

Description

ELISA Kit Technical ManualMSDS

Aspartate Aminotransferase (AST or SGOT) Activity Colorimetric Assay Kit

Aspartate aminotransferase (AST), also known as Glutamate-oxaloacetate transaminase (GOT) is a transaminase (EC 2.6.1.1) similar to the more liver specific alanine transaminase (ALT). Although commonly included clinically as part of a diagnostic liver function test, AST has a broader clinical utility since it may also be elevated in diseases affecting other organs, such as the heart or muscles in myocardial infarction, also in acute pancreatitis, acute hemolytic anemia, severe burns, acute renal disease, musculoskeletal diseases and trauma. It catalyzes the reaction: Aspartate + Ketoglutarate Oxaloacetate + Glutamate Diagnostically, it is almost always measured in units/liter (U/L). In Assay Genie's AST Assay Kit, an amino group is transferred of from aspartate to ketoglutarate. The products of this reversible transamination reaction are oxaloacetate and glutamate. The glutamate is detected in a reaction that concomitantly converts a nearly colorless probe to color (ax = 450 nm). The kit provides a rapid, simple, sensitive and reliable test suitable as a high throughput activity assay of AST with a detection limit of 10 mU per well.

Figure: a) Glutamate Standard Curve obtained using Kit Protocol b) Standard Curve using Positive Control

Key Information Description

Product SKU

BN00966

Size

100 assays

Detection Method

Absorbance (450 nm)

Species Reactivity

Mammalian

Applications

This assay detects AST/ SGOT with a detection limit of 10 mU per well.

Features and Benefits

  • Simple procedure
  • Fast, reliable and convenient
  • Sensitive assays for measuring AST/ SGOT activity in various biological samples and the kit is also suitable for high throughput analyses.

Kit Components

  • AST Assay Buffer
  • AST Enzyme Mix
  • Developer
  • AST Substrate
  • Glutamate Standard (0.1M)
  • AST Positive Control

Storage Conditions

-20°C

Shipping Conditions

Gel Pack

USAGE

For Research Use Only! Not For Use in Humans

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Additional Information

Product type:
Cellular Assay
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