Beta Galactosidase (Beta-Gal) Activity Assay Kit (Fluorometric) (BN01038)

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BN01038
  • Beta Galactosidase (Beta-Gal) Activity Assay Kit (Fluorometric) (BN01038)
  • Beta Galactosidase (Beta-Gal) Activity Assay Kit (Fluorometric) (BN01038)
  • Beta Galactosidase (Beta-Gal) Activity Assay Kit (Fluorometric) (BN01038)
€577

Description

ELISA Kit Technical ManualMSDS

Beta Galactosidase (Beta-Gal) Activity Assay Kit (Fluorometric)

Beta Galactosidase (Beta-Gal, EC: 3.2.1.23) is an enzyme which hydrolyzes the Beta-galactosides into monosacchanides. Beta-Gal is widely used as a reporter gene in the field of molecular biology. Senescence Associated Beta-Gal (SA-Beta-Gal) is an isoform of Beta-Gal which has the optimal activity at pH 6.0, and is mostly used as a biomarker for senescent cells. Beta-Gal is an essential enzyme in humans and its deficiency results in Morquio's Syndrome, a severe birth defect. Assay Genie's Beta Galactosidase Activity Assay kit provides a quick and easy way for monitoring Beta Galactosidase activity in a variety of samples. In this kit, Beta Galactosidase hydrolyses a non-fluorescent substrate to generate a strong fluorescent product. The assay is simple, sensitive, and high-throughput adaptable. Detection limit: < 0.1 mU.

Figure: (a) Fluorescein Standard Curve, (b) Measurement of Beta Galactosidase activity in Yogurt (8 μg), rat spleen (8 μg), and WBC (6 μg) homogenate and (c) Beta Galactosidase specific activity of samples mentioned in Fig. b. Assay was performed according to the kit protocol.

Key Information Description

Product SKU

BN01038

Size

100 assays

Detection Method

Fluorescence (Ex/Em = 480/520 nm)

Applications

Measurement of Beta-Galactosidase activity in various samples

Features and Benefits

  • Quick and easy method to measure Beta Galactosidase activity.
  • Detection limit: < 0.1 mU.

Kit Components

  • Beta-Gal Assay Buffer
  • Beta-Gal Substrate (in DMSO)
  • Fluorescein Standard (1 mM)
  • Beta-Gal Positive Control

Storage Conditions

-20°C

Shipping Conditions

Gel Pack

USAGE

For Research Use Only! Not For Use in Humans.

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