Beta-Lactamase Activity Colorimetric Assay Kit (384-well) (BN01115)

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BN01115
  • Beta-Lactamase Activity Colorimetric Assay Kit (384-well) (BN01115)
  • Beta-Lactamase Activity Colorimetric Assay Kit (384-well) (BN01115)
  • Beta-Lactamase Activity Colorimetric Assay Kit (384-well) (BN01115)
€828

Description

ELISA Kit Technical ManualMSDS

Beta-Lactamase Activity Colorimetric Assay Kit (384-well)

Beta-Lactamases (BetaLs), are a large family of hydrolases comprising more than 850 identified members expressed in Gram-positive and Gram-negative bacteria. BetaLs can be classified according to their substrate or inhibitor specificity. These enzymes are capable of hydrolyzing four atom rings known as Beta-lactams. Antibiotics containing Beta-lactam rings (i.e. penicillin, cephalosporin, monobactam, carbapenem) are highly susceptible to be hydrolyzed via enzymatic activity, which deactivates their antibiotic potency. BetaLs have become a significant clinical threat due to the alarming number of cases of bacterial strains showing Beta-lactam antibiotic resistance. Approaches for combatting this type of resistance have opened up the possibility of developing a new form of Beta lactam antibiotics that are more resistant to Beta-Lactamase enzymatic activity. Therefore, experimental research has been arising in wide extent for developing new drugs or inhibitors effective against the Beta-lactam antibiotic resistance bacteria. Assay Genie's Beta-Lactamase Activity Assay Kit offers a simple and sensitive assay that can detect and quantify the enzymatic activity of these hydrolases. The assay is based on the hydrolysis of Nitrocefin, a chromogenic cephalosporin, that results in the generation of a colored product (OD: 490 nm), which is directly proportional to the amount of BetaL activity. The assay can detect enzymatic activity as low as 15 mU in a variety of biological samples

Figure: a) Nitrocefin Standard Curve. b) βL activity in E. coli H ( E. coli strain with high βL activity) and E.coli L ( E. coli strain with low βL activity). Samples were prepared following the kit’s protocol then 1 µl for E. coli H and 10 µl of E.coli L were added to the well for the assay. Positive Control (2 µl) was added after diluting following the kit’s protocol. Background control for Nitrocefin was prepared according to the kit’s protocol. c) βL Activity of E.coli both higher and lower βL activity strains of E.coli.

Key Information Description

Product SKU

BN01115

Size

400 assays

Detection Method

Absorbance (490 nm)

Applications

  • Measurement of Beta-Lactamase activity in various biological samples
  • Analysis of Beta-Lactamase activity in pathological conditions

Features and Benefits

The assay can detect enzymatic activity as low as 15 mU in a variety of biological samples

Kit Components

  • BetaL Assay Buffer
  • Nitrocefin (in DMSO)
  • Positive Control (Lyophilized)
  • BetaL Hydrolysis Buffer

Storage Conditions

-20°C

Shipping Conditions

Gel Pack

USAGE

For Research Use Only! Not For Use in Humans

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