Beta-Lactamase Inhibitor Screening Kit (Colorimetric) (BN01020)

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ELISA Kit Technical ManualMSDS

Beta-Lactamase Inhibitor Screening Kit (Colorimetric)

Beta-Lactamase (BetaL), (EC, is a bacterial enzyme that hydrolyzes the Beta-Lactam ring, a four-carbon ring, the building block of Beta-Lactam antibiotics. Beta-Lactams inhibit transpeptidases, enzymes that participate in the biosynthesis of bacterial cell walls. Penams, Carbapenems, Cephems, Clavams, Oxacephems and Monobactams have been widely used for the treatment of bacterial infections since the discovery of penicillin, the first Beta-Lactam antibiotic, and its bactericidal effect more than eighty years ago. However, an alarming number of cases of Beta-Lactam antibiotic-resistant infections have been reported. The resistance is found in bacterial strains producing Beta-Lactamase, which is responsible for diminishing the Beta-Lactam antibiotic potency. Beta-Lactamase inhibition, therefore, has become an urgent target in the treatment of bacterial infections displaying Beta-Lactam resistance. Several Beta-Lactam derivatives have been reported as Beta-Lactamase inhibitors; however, only clavulanic acid, sulbactam and tazobactam have reached clinical importance. Assay Genie's Beta-Lactamase Inhibitor Screening Kit utilizes the ability of the enzyme to hydrolyze Nitrocefin, a well-known Beta-Lactamase substrate. In the presence of Clavulanic Acid-a potent inhibitor of Beta-Lactamase, the rate of hydrolysis of the substrate is decreased. The kit provides a rapid, simple, sensitive, and reliable test suitable for high- throughput screening of Beta-Lactamase inhibitors.

Figure: Inhibition of β-Lactamase Enzymatic Activity with Clavulanic Acid. Assay was performed following kit protocol.

Key Information Description

Product SKU



100 assays

Detection Method

Absorbance (490 nm)


Screening/studying/characterizing Beta-Lactamase Inhibitors for efficient treatment of antibiotic resistant bacterial infections

Features and Benefits

  • Rapid, simple & sensitive
  • High-throughput suitable
  • Includes Inhibitor Control (Clavulanic Acid)

Kit Components

  • Beta-Lactamase Assay Buffer
  • Nitrocefin (in DMSO)
  • Beta-Lactamase (Lyophilized)
  • Inhibitor Control (Clavulanic Acid)

Storage Conditions


Shipping Conditions

Gel Pack


For Research Use Only! Not For Use in Humans.

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