Beta-N-Acetylglucosaminidase Activity Assay Kit (Colorimetric) (BN00952)

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BN00952
  • Beta-N-Acetylglucosaminidase Activity Assay Kit (Colorimetric) (BN00952)
  • Beta-N-Acetylglucosaminidase Activity Assay Kit (Colorimetric) (BN00952)
  • Beta-N-Acetylglucosaminidase Activity Assay Kit (Colorimetric) (BN00952)
€565

Description

ELISA Kit Technical ManualMSDS

Beta-N-Acetylglucosaminidase Activity Assay Kit (Colorimetric)

Beta-N-Acetylglucosaminidase (NAG, EC 3.2.1.52) is a lysosomal enzyme that is expressed in various tissues, including kidney, liver and lungs. NAG can cleave N-acetyl-glucosamine, a monosaccharide derivative of glucose. Its concentration in urine is minimal due to its inability to cross the glomerular basal membrane. Increased concentration of NAG in urine indicates renal tubular cell breakdown. Acute Kidney Injury (AKI) is the sudden loss of kidney functions, causing electrolyte imbalance, and retention of urea and other nitrogenous products. NAG has become one of the most studied and used biomarkers for the detection and diagnosis of AKI. Assay Genie's NAG Activity Assay Kit provides a simple and sensitive method for monitoring NAG enzymatic activity. In this assay, NAG uses a synthetic p-nitrophenol derivative (R-pNP) as a NAG substrate and releases pNP which can be measured at absorbance (OD 400 nm). The assay can detect as low as 50 µU of NAG activity in a variety of samples.

Figure: (a) pNP Standard Curve. (b) Measurement of NAG activity in human urine from different donors. Undiluted samples (70 µl) were incubated for 30 min. with NAG substrate. (c) Measurement of NAG activity in mouse kidney (10 µg) and human serum (20 µl). Samples were incubated for 30 min. with NAG substrate. All assays were performed following the kit protocol.

Key Information Description

Product SKU

BN00952

Size

100 assays

Detection Method

Absorbance (400 nm)

Applications

Measurement of NAG in biological samples from different mammalian species

Features and Benefits

  • Convenient & sensitive
  • The assay can detect as low as 50 µU of NAG activity in a variety of samples

Kit Components

  • NAG Assay Buffer
  • NAG Substrate
  • p-Nitrophenol (pNP) (20 mM)
  • NAG Stop Solution
  • NAG Positive Control

Storage Conditions

-20°C

Shipping Conditions

Gel Pack

USAGE

For Research Use Only! Not For Use in Humans.

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