Beta-Secretase Activity Fluorometric Assay Kit (BN00623)

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SKU:
BN00623
  • Beta-Secretase Activity Fluorometric Assay Kit (BN00623)
  • Beta-Secretase Activity Fluorometric Assay Kit (BN00623)
€708

Description

ELISA Kit Technical ManualMSDS

Beta-Secretase Activity Fluorometric Assay Kit

Beta-Secretase has been implicated to be an excellent target for anti-amyloid therapy for the treatment of Alzheimer's disease. The Beta-Secretase activity Assay Kit provides a convenient fluorescence method for detecting Beta-secretase activity in biological and purified samples. The assay utilizes a secretase-specific peptide conjugated to two reporter molecules EDANS and DABCYL. In the uncleaved form, the fluorescent emissions from EDANS are quenched by the physical proximity of the DABCYL moiety. Cleavage of the peptide by secretase physically separates EDANS and DABCYL allowing for the release of a fluorescent signal. The level of secretase enzymatic activity in samples is proportional to the level of fluorescence intensity.

Key Information Description

Product SKU

BN00623

Size

100 assays

Detection Method

Fluorescence (Ex/Em 335-355/ 495-510 nm)

Species Reactivity

Mammalian

Applications

  • Convenient fluorescence method for detecting
  • Beta-secretase activity in biological and purified samples.

Features and Benefits

  • Simple procedure; takes ~ 1 hour
  • Fast and convenient
  • The assay is sensitive, stable and high-throughput adaptable.

Kit Components

  • Beta-Secretase Extraction Buffer
  • Beta-Secretase Reaction Buffer (2X)
  • Beta-Secretase Substrate (in DMSO)
  • Active Beta-Secretase (Lyophilized)
  • Beta-Secretase Inhibitor (in DMSO)

Storage Conditions

-20°C

Shipping Conditions

Gel Pack

USAGE

For Research Use Only! Not For Use in Humans

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