BrdU Cell Proliferation Assay Kit (BN00564)

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BN00564
€1,044

Description

ELISA Kit Technical ManualMSDS

BrdU Cell Proliferation Assay Kit

5-bromo-2-deoxyuridine (BrdU) is a pyrimidine analog. It gets incorporated into the newly synthesized DNA of proliferating cells in place of thymidine. Assay Genie's BrdU Cell Proliferation Assay Kit detects incorporated BrdU using a mouse anti-BrdU antibody. An anti-mouse HRP-linked secondary antibody is used to detect the anti-BrdU antibody bound to BrdU, which is followed by addition of TMB (a HRP substrate). The extent of color development is proportional to the quantity of BrdU incorporated into the cells and can be used directly as an indicator of cell proliferation. Compared to other cell proliferation assays, this kit detects only the proliferating cells and not the seeded cells. This highly sensitive, non-radioactive kit detects as less as 50-100 proliferating cells.

Figure: Treatment of Balb/3T3cells with h-FGF2 increases cell proliferation as detected by BrdU Cell Proliferation Assay. The cells were seeded at 5000 cells/well in a 96-well plate and incubated overnight at 37°C followed by an overnight incubation in serum-free media at 37°C. h-FGF2 was then added into the plate at different concentrations and cells were incubated for 24 hrs at 37°C. 1X BrdU solutionnwas added into each well and the cells were further incubated for 4 hrs.

Key Information Description

Detection Method

Absorbance (450 nm)

Size Options

  • 200 Assays
  • 1000 Assays

Species Reactivity

N/A

Applications

Detection and quantification of cell proliferation induced by growth factors, cytokines, mitogens, and nutrients.Analysis of cytotoxic and cytostatic compounds such as anticancer drugs, toxic agents and other pharmaceuticals.Determination of the inhibitory or stimulatory effects of various compounds on cell proliferation.

Features and Benefits

  • Convenient & non-radioactive
  • High-throughput suitable
  • Highly-sensitive: detect as low as 50-100 proliferating cells.
  • Suitable for cell proliferation, chemotaxis and cytotoxicity

Kit Components

  • BrdU (1000X)
  • Fixing/Denaturing Solution
  • BrdU Detection Antibody (300X)
  • Anti-mouse HRP-linked Antibody (2000X)
  • Antibody Diluent
  • Wash Buffer (10X)
  • TMB Substrate
  • Stop Solution

Storage Conditions

-20°C

Shipping Conditions

Gel Pack

USAGE

For Research Use Only! Not For Use in Humans

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