Caspase-1 Fluorometric Assay Kit

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SKU:
BN00024
€290

Description

ELISA Kit Technical ManualMSDS

Caspase-1 Fluorometric Assay Kit

Activation of ICE-family proteases/caspases initiates apoptosis or other cellular processes in mammalian cells. The Caspase-1/ICE Fluorometric Assay Kit provides a simple and convenient means for assaying the activity of caspases that recognize the sequence YVAD. The assay is based on detection of cleavage of substrate YVAD-AFC (AFC: 7-amino-4-trifluoromethyl coumarin). YVAD-AFC emits blue light (400 nm); upon cleavage of the substrate by caspase-1 or related caspases, free AFC emits a yellow-green fluorescence (505 nm), which can be quantified using a fluorometer or a fluorecence microtiter plate reader. Comparison of the fluorescence of AFC from a treated sample with an untreated control allows determination of the fold increase in caspase-1 activity.

Key Information Description

Detection Methods

Fluorescence (400 nm excitation filter and 505 nm emission filter)

Size Options

  • 25 Assays
  • 100 Assays
  • 200 Assays
  • 400 Assays

Species Reactivity

Mammalian

Applications

Detect early/middle stages of apoptosis; differentiate apoptosis from necrosis.

Features and Benefits

  • Simple one-step procedure; takes 1-2 hours
  • Fast and convenient
  • Comparison of the fluorescence of AFC from a treated sample with an untreated control allows determination of the fold increase in caspase-1 activity

Kit Components

  • Cell Lysis Buffer
  • 2X Reaction Buffer
  • YVAD-AFC (1mM)
  • DTT (1 M)

Storage Conditions

-20°C

Shipping Conditions

Gel Pack

USAGE

For Research Use Only! Not For Use in Humans.

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