Caspase-12 Fluorometric Assay Kit (BN00339)



ELISA Kit Technical ManualMSDS

Caspase-12 Fluorometric Assay Kit

Caspase family of proteases are the central mediators of apoptosis in mammalian cells. The Caspase-12 Fluorometric Assay Kit provides a simple and convenient means for assaying the activity of caspases that recognize the sequence ATAD. The assay is based on detection of cleavage of substrate ATAD-AFC (AFC: 7-amino-4-trifluoromethyl coumarin). ATAD-AFC emits blue light (ax = 400 nm); upon cleavage of the substrate by caspase-12 or related caspases, free AFC emits a yellow-green fluorescence (ax = 505 nm), which can be quantified using a fluorometer or a fluorecence microtiter plate reader.

Key Information Description

Detection Method

Fluorescence (Ex/Em 400/505)

Size Options

  • 25 Assays
  • 100 Assays

Species Reactivity



Detect early/middle stages of apoptosis; differentiate apoptosis from necrosis.

Features and Benefits

  • Simple one-step procedure; takes only 1-2 hours
  • Fast and convenient
  • Comparison of the fluorescence of AFC from an apoptotic sample with an uninduced control allows determination of the fold increase in caspase-12 activity.

Kit Components

  • Cell Lysis Buffer
  • 2X Reaction Buffer
  • ATAD-AFC Substrate
  • DTT (1 M)

Storage Conditions


Shipping Conditions

Gel Pack


For Research Use Only! Not For Use in Humans.

View AllClose

Additional Information

Product type:
Cellular Assay
View AllClose