Caspase-5 Fluorometric Assay Kit (BN00075)

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BN00075
€283

Description

ELISA Kit Technical ManualMSDS

Caspase-5 Fluorometric Assay Kit

Activation of ICE-family proteases/caspases initiates apoptosis in mammalian cells. The Caspase-5 Fluorometric Assay Kit provides a simple and convenient means for assaying the activity of caspase-5 and related caspases that recognize the sequence WEHD. The assay is based on detection of cleavage of substrate WEHD-AFC (AFC: 7-amino-4-trifluoromethyl coumarin). WEHD-AFC emits blue light (ax = 400 nm); upon cleavage of the substrate by caspase-5 or related caspases, free AFC emits a yellow-green fluorescence (ax = 505 nm), which can be quantified using a fluorometer or fluorecence microtiter plate reader.

Key Information Description

Detection Method

Fluorescence (Ex/Em 400/505 nm)

Species Reactivity

Mammalian

Size

  • 25 Assays
  • 100 Assays
  • 200 Assays
  • 400 Assays

Applications

Detect early/middle stages of apoptosis; differentiate apoptosis from necrosis.

Features and Benefits

  • Simple one-step procedure; takes only 1-2 hours
  • Fast and convenient
  • Comparison of the fluorescence of AFC from a treated sample with an untreated control allows determination of the fold increase in caspase-5 activity.

Kit Components

  • Cell Lysis Buffer
  • 2X Reaction Buffer
  • WEHD-AFC (1 mM)
  • DTT (1 M)

Storage Conditions

-20°C

Shipping Conditions

Gel Pack

USAGE

For Research Use Only! Not For Use in Humans.

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