Catalase Activity Colorimetric/Fluorometric Assay Kit (BN00986)

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SKU:
BN00986
  • Catalase Activity Colorimetric/Fluorometric Assay Kit (BN00986)
  • Catalase Activity Colorimetric/Fluorometric Assay Kit (BN00986)
€487

Description

ELISA Kit Technical ManualMSDS

Catalase Activity Colorimetric/Fluorometric Assay Kit

Catalase (EC 1.11.1.6) is a ubiquitous antioxidant enzyme that is present in nearly all living organisms. It functions to catalyze the decomposition of hydrogen peroxide (H to water and oxygen. Assay Genie's Catalase Assay Kit provides a highly sensitive, simple, direct and HTS-ready assay for measuring Catalase activity in biological samples. In the assay, catalase first reacts with Hto produce water and oxygen, the unconverted Hreacts with GenieRed probe to produce a product, which can be measured at 570 nm (Colorimetric method) or at Ex/Em=535/587nm (fluorometric method). Catalase activity is reversely proportional to the signal. The kit detects high pico-unit of catalase in samples.

(a)

(b)

Figure: (a) H2O2 Standard Curve obtained using kit protocol. (b) Histogram of sample tests comparing high control vs sample.

Key Information Description

Product SKU

BN00986

Size

100 assays

Detection Method

Absorbance (570 nm) or Fluorescence (Ex/Em 535/587 nm)

Species Reactivity

Mammalian

Applications

The kit detects high pico-unit of catalase in samples.

Features and Benefits

  • Simple procedure; takes ~ less than 40 minutes
  • Fast and convenient

Kit Components

  • Catalase Assay Buffer
  • GenieRed Probe (in DMSO)
  • HRP (lyophilized)
  • H (3%; 0.88M)
  • Stop Solution
  • Catalase Positive Control (lyophilized)

Storage Conditions

4°C

Shipping Conditions

Gel Pack

USAGE

For Research Use Only! Not For Use in Humans.

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