Cathepsin E Activity Assay Kit (Fluorometric) (BN00445)

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BN00445
  • Cathepsin E Activity Assay Kit (Fluorometric) (BN00445)
  • Cathepsin E Activity Assay Kit (Fluorometric) (BN00445)
€726

Description

ELISA Kit Technical ManualMSDS

Cathepsin E Activity Assay Kit (Fluorometric)

Cathepsin E (CTSE, EC: 3.4.23.34) is a gastric aspartyl protease that functions as a disulfide-linked homodimer. This protease has a specificity similar to that of pepsin A and cathepsin D. It is an intracellular proteinase that is found in highest concentration on the surface of epithelial mucus-producing cells of the stomach. It is the first aspartic proteinase expressed in the fetal stomach and is found in more than half of gastric cancers. Assay Genie's Cathepsin E Activity Assay Kit utilizes the ability of an active Cathepsin E to cleave a synthetic MCA-based peptide substrate to release free MCA, which can be easily quantified using a fluorometer or fluorescence microplate reader. This assay kit is simple, rapid and can detect Cathepsin E activity as low as 1 mU in biological samples.

Figure: (a) MCA Standard Curve, (b) Cathepsin E activity in rat liver lysate (6 µg), rat kidney lysate (12 µg), and Positive Control (2 µl).
Assays were performed following the kit protocol.

Key Information Description

Product SKU

BN00445

Size

100 assays

Detection Method

Fluorescence (Ex/Em = 320/420 nm)

Applications

  • Detection of Cathepsin E activity in tissue and cell lysates
  • Determine activity of purified Cathepsin E

Features and Benefits

  • Rapid & convenient
  • Detects Cathepsin E activity as low as 1 mU in biological samples

Kit Components

  • CTSE Assay Buffer
  • CTSE Lysis Buffer
  • CTSE Substrate
  • Human Cathepsin E (Positive Control)
  • MCA Standard (1 mM)

Storage Conditions

-20°C

Shipping Conditions

Gel Pack

USAGE

For Research Use Only! Not For Use in Humans

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