Cell Invasion Assay (Collagen I), 96-well, 8 µm (BN01090)

(No reviews yet) Write a Review
SKU:
BN01090
  • Cell Invasion Assay (Collagen I), 96-well, 8 µm (BN01090)
  • Cell Invasion Assay (Collagen I), 96-well, 8 µm (BN01090)
  • Cell Invasion Assay (Collagen I), 96-well, 8 µm (BN01090)
€661

Description

ELISA Kit Technical ManualMSDS

Cell Invasion Assay (Collagen I), 96-well, 8 µm

Cell invasion is the ability of cells to migrate from one area to another through an extracellular matrix. Cell invasion is exhibited by both normal cells as well as cancerous cells in response to specific external signals, including chemical & mechanical stimuli. During invasion, extracellular matrix is enzymatically degraded by cellular proteases before cells migrate to the new location. Cell invasion is required for normal processes such as wound repair, vasculature formation and the inflammatory response as well as the abnormal invasion of tissues by tumor cells during metastasis. Assay Genie's Cell Invasion Assay Kit utilizes a Boyden chamber coated with Collagen I, where the cells invade the matrix and then migrate through a semipermeable membrane in the Boyden chamber in response to stimulants or inhibitory compounds. The percent cell invasion can be analyzed directly in a plate reader. Our assay is easy to use, sensitive and adaptable to high-throughput systems.

Figure 1: (a) Cell Invasion plate: The cells are added to the Top Chamber and the Control Invasion Inducer or chemoattractant are added to the Bottom Chamber. (b) Standard Curve: HT-1080 cells were harvested, counted and serially diluted to obtain desired cell number. Cells were incubated according to the protocol. (c) Cell Invasion: NIH-3T3 and HT-1080 cells were starved overnight and treated with Control (Cnt) Invasion Inducer or remain untreated (No Treatment). Treatment with Control Invasion Inducer demonstrated a significant increase in invasion of HT 1080 cells as compared to NIH-3T3 control cells.

Key Information Description

Product SKU

BN01090

Size

100 assays

Detection Method

Fluorescence (Ex/Em = 485/530 nm)

Species Reactivity

Mammalian cells

Applications

  • Measure cell invasion in response to stimuli
  • Screen and characterize compounds that influence cell invasion

Features and Benefits

  • Highly sensitive fluorometric method to measure cell invasion in response to a variety of biochemical stimuli
  • Simple & High throughput-adaptable
  • Reproducible, Quantitative tool for screening, studying, and characterizing compounds that affect cell invasion

Kit Components

  • Wash Buffer
  • Cell Dissociation Solution
  • Control Invasion Inducer
  • Cell Dye
  • Cell Invasion Chamber
  • Collagen I

Storage Conditions

-20°C

Shipping Conditions

Gel Pack

USAGE

For Research Use Only! Not For Use in Humans.

View AllClose