Chloride Colorimetric Assay Kit (BN00762)

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ELISA Kit Technical ManualMSDS

Chloride Colorimetric Assay Kit

Chloride is the anionic form of chlorine. It is the most common of the anions found in living organisms. Chloride ions play a variety of important physiological roles. Chloride channels are found in a variety of cells and are responsible for setting resting cell membrane potential and regulating cell volume. In the nervous system, the action of glycine and GABA are related to chloride levels in specific neurons. Chloride is also instrumental in maintaining the acid-base balance in blood. The kidneys are instrumental in closely regulating serum chloride levels. There are a number of pathologies associated with defective chloride transport; the most well-known being Cystic Fibrosis, caused by a mutation in CFTR a membrane chloride transporter. Assay Genie's Chloride Assay Kit provides a quick, simple method for quantification of Chloride in a variety of biological samples. Blood and urine can be used directly after dilution with water. The assay is based upon the competition of Hg²and Fe²for TPTZ. The preferred Hg-TPTZ adduct exhibits no color. In the presence of Chloride, Hg²forms HgCL2 freeing up TPTZ which then binds the available Fe²giving a very intense absorbance with a ax~ 620nm. The assay is linear in the range 20 to 120 nmol Chloride/well with detection sensitivity ~0.4 mM chloride.

Chloride Standard Curve: Assays were performed following the kit protocol.

Key Information Description

Product SKU



100 assays

Detection Method

Absorbance (620 nm)

Species Reactivity



The assay is linear in the range 20 to 120 nmol Chloride/well with detection sensitivity ~0.4 mM chloride.

Features and Benefits

  • Simple procedure; takes less than ~20 minutes
  • Fast and convenient
  • Mn² Cu² Zn²do not interfere with the assay.

Kit Components

  • Chloride Reagent
  • Chloride Standard (10 µmol)

Storage Conditions


Shipping Conditions



For Research Use Only! Not For Use in Humans.

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Additional Information

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