Chloroplast Isolation Kit (BN00703)

SKU:
BN00703
  • Cell Biology Assays Chloroplast Isolation Kit BN00703
  • Cell Biology Assays Chloroplast Isolation Kit BN00703
  • Cell Biology Assays Chloroplast Isolation Kit BN00703
  • Cell Biology Assays Chloroplast Isolation Kit BN00703
€637

Description

ELISA Kit Technical ManualMSDS

Chloroplast Isolation Kit

Chloroplasts are membrane-bound organelles found in plant and algal cells which perform the function of photosynthesis. Plant chloroplasts are lens-shaped, usually 5-10 µm in diameter and 1-3 µm thick. Although leaves contain the majority of plants' chloroplasts, they can also be found in stems, seeds, and un-ripened fruit. Assay Genie's Chloroplast Isolation Kit provides an easy method to isolate chloroplast from a broad range of plant types including dicots, monocots, and conifers. Isolated chloroplasts can be used to study photosynthetic processes and as starting materials for chloroplast membrane, protein, chloroplast DNA and chloroplast RNA isolations

Figure: (a) Light microscope image of Chloroplasts Isolated from Ficus leaves; (b) Representation of a Chloroplast Gradient isolation of intact chloroplasts; (c) Chlorophyll a content at each processing step: a: debris pellet, first centrifugation (Step 1-f); b: supernatant from chloroplast (Step 1-g); c: Intact chloroplast layer (Step 2-c); d: broken chloroplast layer (Step 2-c); (d) Chlorophyll a content of isolated chloroplasts from multiple species: a: Spider Plant (Chlorophytum comosum); b: Corn husks (Zea mays); c: Pine needles (Pinus muricata); d: Cypress needles (Cupressus sempervirens); e: Juniper (Juniperus species); f: Lettuce (Lactuca sativa); g: Stapelia variegata; h: Ficus species.

Key Information Description

Product SKU

BN00703

Size

10 g

Detection Method

  • Isolation of high purity chloroplasts from leaves/plant tissues.
  • Study photosynthetic processes, chloroplast membranes, thylakoid membranes, and associated proteins

Species Reactivity

Plant

Applications

  • Isolation of high purity chloroplasts from leaves/plant tissues.
  • Study photosynthetic processes, chloroplast membranes, thylakoid membranes, and associated proteins

Features and Benefits

Simple & rapid method to isolate highly pure, intact and functional mitochondria from tissues and cultured cells.

Kit Components

  • Chloroplast Isolation Buffer, 2X
  • BSA (10% solution)
  • DTT (1 M)
  • Opti-Prep Density Gradient Medium

Storage Conditions

-20°C

Shipping Conditions

Gel Pack

USAGE

For Research Use Only! Not For Use in Humans.

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Additional Information

Product type:
Cellular Assay
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