Cholesterol Detection Kit (cell-based) (BN00815)

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BN00815
  • Cholesterol Detection Kit (cell-based) (BN00815)
  • Cholesterol Detection Kit (cell-based) (BN00815)
  • Cholesterol Detection Kit (cell-based) (BN00815)
€392

Description

ELISA Kit Technical ManualMSDS

Cholesterol Detection Kit (cell-based)

Cholesterol is an essential structural component of animal cell membranes. It is required to maintain membrane structural integrity and fluidity. Cholesterol also serves as a precursor for the biosynthesis of several hormones, bile acid and vitamin D. Plasma membrane contains majority of the cellular cholesterol (80-90%) whereas little cholesterol resides in endoplasmic reticulum and in mitochondrial membrane. Transport of intracellular cholesterol within the cells to different compartments is through vesicular and non-vesicular pathways. Defects in these transport processes can alter cellular cholesterol metabolism resulting in pathological conditions. Filipin III is widely used as a probe for sterol localization in membranes. Interaction with cholesterol alters Filipin absorption and fluorescence spectra. This assay provides a simple, easy to perform, histochemical method of identification of unesterified cholesterol.

Figure 1: Liver cells (HepG2) cholesterol staining: HepG2 cells were cultured overnight and next day treated with U-18666A (1.25 uM) or vehicle control (DMSO) for 48 hrs. (a) Cells treated with DMSO alone demonstrated majority of the cholesterol is localized in Plasma membrane. (b) Treatment with U-18666A for 48 hrs resulted in intracellular accumulation of cholesterol. (c) Higher magnification of image B. Images were taken using Nikon TiE microscope.

Key Information Description

Product SKU

BN00815

Size

100 assays

Detection Method

Light and Fluorescence Microscopy (Ex/Em = 340-380/385-470 nm).

Applications

Screen/study/characterize stimulators/inhibitors that affect cholesterol transport and localization in a variety of cells.

Features and Benefits

  • Simple, easy to perform, histochemical method of identification of unesterified cholesterol.
  • Includes U-18666A, a cholesterol transport inhibitor.

Kit Components

  • Fixative Solution
  • Assay Buffer
  • Staining Dye

Storage Conditions

-20°C

Shipping Conditions

Gel Pack.

USAGE

For Research Use Only! Not For Use in Humans.

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