Cobalt Colorimetric Assay Kit (BN00736)

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SKU:
BN00736
€529

Description

ELISA Kit Technical ManualMSDS

Cobalt Colorimetric Assay Kit

Cobalt is a hard, lustrous, grey metal, a chemical element (symbol Co, atomic number 27 and atomic mass 58.9). Cobalt is an essential trace-element for all multicellular organisms as the active center of coenzymes called cobalamins. These include vitamin B-12 which is essential for plants and animals. Cobalt is also an active nutrient for bacteria, algae, and fungi, and may be a necessary nutrient for all life. Cobalt, particularly the Co²species, forms complexes with a large number of organic and inorganic molecules with a range of extinction coefficients in the visible range. The Assay Genie Cobalt Assay kit takes advantage of the reaction of Co²with mercaptoethanol in basic media. Under the condition, Cobalt forms a complex with a strong absorbance band at 475 nm. The metal ions Fe² Cu² Ni² Zn² Mn² exhibit less than 10% interference at this wavelength. The kit provides a quick simple accurate method of quantitating Co²in a variety of samples.

Cobalt Standard Curve: Assays were performed following the kit protocol.

Key Information Description

Product SKU

BN00736

Size

100 assays

Detection Method

Absorbance (475 nm)

Species Reactivity

Mammalian

Applications

The assay is linear in the range 3 to 50 nmol (0.18-3 µg) Cobalt, with a detection limit of ~ 3 nmol (or ~15 µM, 0.18 µg).

Features and Benefits

  • Simple procedure; takes only 20 minutes
  • Fast and convenient
  • The Assay Genie Cobalt Assay kit takes advantage of the reaction of Co²with mercaptoethanol in basic media.

Kit Components

  • Cobalt Reagent
  • Cobalt Chloride Standard (1.0 µmol)

Storage Conditions

-20°C

Shipping Conditions

Gel Pack

USAGE

For Research Use Only! Not For Use in Humans

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Additional Information

Product type:
Assay
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