Description
Cobalt Colorimetric Assay Kit
Cobalt is a hard, lustrous, grey metal, a chemical element (symbol Co, atomic number 27 and atomic mass 58.9). Cobalt is an essential trace-element for all multicellular organisms as the active center of coenzymes called cobalamins. These include vitamin B-12 which is essential for plants and animals. Cobalt is also an active nutrient for bacteria, algae, and fungi, and may be a necessary nutrient for all life. Cobalt, particularly the Co²species, forms complexes with a large number of organic and inorganic molecules with a range of extinction coefficients in the visible range. The Assay Genie Cobalt Assay kit takes advantage of the reaction of Co²with mercaptoethanol in basic media. Under the condition, Cobalt forms a complex with a strong absorbance band at 475 nm. The metal ions Fe² Cu² Ni² Zn² Mn² exhibit less than 10% interference at this wavelength. The kit provides a quick simple accurate method of quantitating Co²in a variety of samples.
Cobalt Standard Curve: Assays were performed following the kit protocol.
Key Information | Description |
Product SKU | BN00736 |
Size | 100 assays |
Detection Method | Absorbance (475 nm) |
Species Reactivity | Mammalian |
Applications | The assay is linear in the range 3 to 50 nmol (0.18-3 µg) Cobalt, with a detection limit of ~ 3 nmol (or ~15 µM, 0.18 µg). |
Features and Benefits |
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Kit Components |
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Storage Conditions | -20°C |
Shipping Conditions | Gel Pack |
USAGE | For Research Use Only! Not For Use in Humans |