Collagenase Activity Colorimetric Assay Kit (BN01008)

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BN01008
  • Collagenase Activity Colorimetric Assay Kit (BN01008)
  • Collagenase Activity Colorimetric Assay Kit (BN01008)
€475

Description

ELISA Kit Technical ManualMSDS

Collagenase Activity Colorimetric Assay Kit

Collagenase (EC 3.4.24.3) is an enzyme in the matrix metalloproteinase family that breaks down collagen, assisting in degradation of the extracellular matrix, a key step in the pathogenesis of bacteria. Collagen is an abundant structural protein present in the connective tissue of animals. Collagenase has been used clinically for the treatment of Dupuytren's contracture, an affliction characterized by a thickening of connective tissue. Assay Genie's Collagenase Activity Assay Kit provides a quick and easy way to determine activity of Collagenase. The Kit measures collagenase activity using a synthetic peptide (FALGPA) that mimics collagen's structure. It is suitable for measuring activity of bacterial collagenases such as from Clostridium histolyticum type I-XI. In addition, it can also be used to screen/characterize collagenase inhibitors. The limit of detection for this assay is 0.02 mU Collagenase.

  (a)

(b)

Figure: (a) Collagenase activity over 10 min. (b) Enzyme activity of collagenase and inhibition by 10 mM (1,10)-Phenanthroline. Assay was performed following the kit protocol.

Key Information Description

Product SKU

BN01008

Size

100 assays

Detection Method

Absorbance (345 nm)

Species Reactivity

Bacterial

Applications

Measurement of collagenase activityFor screening/studying/characterizing collagenase inhibitors

Features and Benefits

  • Rapid & sensitive
  • Simple assay to measure collagenase activity as well as to screen/study/characterize potential inhibitors of collagenase
  • Includes Inhibitor Control (1,10-Phenanthroline)

Kit Components

  • Collagenase Assay Buffer
  • Collagenase (0.35 U/ml)
  • Collagenase Substrate (FALGPA)
  • Inhibitor (1 M 1,10-Phenanthroline)

Storage Conditions

-20°C

Shipping Conditions

Gel Pack

USAGE

For Research Use Only! Not For Use in Humans.

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Additional Information

Product type:
Assay
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