Conc Exosome DNA Extraction Kit (BN00113)

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BN00113
  • Conc Exosome DNA Extraction Kit (BN00113)
  • Conc Exosome DNA Extraction Kit (BN00113)
  • Conc Exosome DNA Extraction Kit (BN00113)
$1,472

Description

ELISA Kit Technical ManualMSDS

Conc Exosome DNA Extraction Kit

Exosomes are small endosome derived lipid nanoparticles (50-120 nm) actively secreted by exocytosis by most living cells. Exosome release occurs either constitutively or upon induction, under both normal and pathological conditions, in a dynamic, regulated and functionally relevant manner. Both the amount and the molecular composition of released exosomes depend on the state of a parent cell. Circulating DNA is emerging as a novel non-invasive tool for patient’s stratification and disease monitoring. While most of the research has focused on circulating cell-free (cfDNA) or circulating-tumor-cell (CTC) derived DNA, extracellular vesicle (EVs) associated DNA (EV-DNA) is emerging as a third valuable “liquid biopsy” platform. Genomic single or double-stranded DNA and mitochondrial DNA have been recently detected in extracellular vesicles. However, the majority of double-stranded DNA seems to be associated with tumor derived exosomes (Thakur BK et al. 2014; Kalhert et al. 2014) where it represents the entire genome of the cancerous tumor from which exosomes were derived. This discovery corroborates the potential of exosomes, which can be easily obtained from a simple blood test. Conc Kit combines the ability of our reagent to co-isolate EVs and circulating DNA from biofluids (plasma, urine, serum etc.) or culture supernatants with a user friendly system for DNA purification. Isolated vesicles are lysed with the appropriate lysis buffer and DNA is purified by spin columns and optimized buffers with a fast turnaround time (≈30 min). Finally, Conc Kit provides an appropriated DNA concentrator for concentrating the yield (4-fold concentration) and increasing the purity of the DNA to the levels required for digital PCR analysis (dPCR).

 

Figure 1: Electropherograms of genomic DNA extracted with ExoGenie Kit with or without DNAse treatment

 

Figure 2: a) Conc Kit (EXO-DNAc) is able to concentrate around 4X the DNA yield extracted with the standard protocol (EXO-DNA). DNA yield measured by Nanodrop. b) Conc Kit (EXO-DNAc) improves the DNA purity, compared to the standard protocol (EXO-DNA). DNA purity is expressed by 260/280 ratio.

 

Figure 3: Detection of the mutation BRAFV600E by digital PCR in genomic DNA isolated from serum of a metastatic prostate cancer patient treated with abiraterone. Circulating and EVs associated DNA was extracted by Conc Kit and a competitor kit. Digital PCR analysis revealed mutations in DNA extracted with Conc, whereas no mutation was detectable in DNA extracted with the competitor kit.

Key Information Description

Detection Method

Isolation and purification of circulating and Exosome-associated genomic DNA from plasma and serum

Size Options

  • 20 Reactions
  • 40 Reactions

Species Reactivity

human plasma, serum

Applications

Isolation and purification of circulating and Exosome-associated genomic DNA from plasma and serum

Features and Benefits

Direct exosome capture and DNA purification from plasma and serum without time consuming purification steps.

Kit Components

  • Exosome Lysis buffer
  • Washing buffer 1
  • Washing buffer 2
  • Elution buffer
  • Columns
  • Elution tubes (1.5 ml)
  • Binding Buffer
  • DNA Column Concentration
  • Lyophilized Exosome Standard (Plasma/Serum)

Storage Conditions

4°C

Shipping Conditions

Gel Pack

USAGE

For Research Use Only! Not For Use in Humans.

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